LDH Cytotoxicity Assay Kit
Katalog-Nummer CA0020
Size : 400assays
Marke : Canvax Biotech
LDH Cytotoxicity Assay Kit
Sensitive and reproducible colorimetric LDH assay for cytotoxicity detection in cultured cells.
LDH Cytotoxicity Assay Kit measures cell membrane integrity by detecting lactate dehydrogenase (LDH) released from damaged cells. It provides a simple and colorimetric method for assessing cytotoxicity and cell viability.
The assay offers high sensitivity and reproducible results, making it suitable for drug testing, toxicology studies, and cell-based research.
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Detailed information:
Advantages & Features
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Accurate: direct correlation with damaged cell number.
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Sensitive: detects even low levels of LDH release.
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Fast protocol: single-step procedure.
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Reproducible: stable enzyme activity ensures consistency.
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Safe: non-radioactive colorimetric assay.
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High-throughput compatible: ideal for screening assays.
Specifications
Includes
Includes for 400 assays:
– 4 x 5 mL LDH Assay Buffer
– 4 x 75 μL Dye solution
– 4 mL Lysis solution
– 20 mL Stop solution
Download documentation
Applications
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Quantification of cell death and cytotoxicity.
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Toxicity testing of compounds, detergents, toxins, and pollutants.
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Evaluation of environmental stress on cultured cells.
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High-throughput cytotoxicity screening.
Tables & Figures
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Quality Control
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Lot-to-lot reproducibility validated.
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Tested for sensitivity and linearity.
Advice
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Protect reagents from light.
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Prepare fresh dye solution for best results.
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Always include appropriate positive and negative controls.
Storage & Shipping
- Shipped in: Gel Pack.
- Storage: LDH Assay Buffer and Dye solution (-20 °C) and Lysis Solution & Stop solution (+4°C).
- Recommendations: Protect the product from light.
Citations
- Carletti, A., Pes, K., Tarasco, M., Rosa, J. T., Poudel, S., Pereira, H., … & Gavaia, P. Immunomodulatory inhibition of osteoclastogenesis by a marine microalgal ethanol fraction targeting T-cells, antigen presentation, and macrophage fate. Frontiers in Immunology, 16, 1655321.
- Gorgogietas, V., Weiss, A., Cousin, L., Hoffmann, D., Schmitt, K., Ogier, A., … & Wilbertz, J. H. (2025). Morphological profiling reveals neuroprotection via mitochondrial uncoupling in human dopaminergic neurons. Scientific Reports, 15(1), 29507.
- López Baltanás, R. (2023). Influencia del suplemento de magnesio sobre el estrés oxidativo, la Inflamación y la disfunción vascular asociada a la enfermedad renal crónica: estudios in vivo e in vitro.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
LDH cytotoxicity assay kit, Lactate dehydrogenase assay, Cell cytotoxicity assay LDH, LDH release assay, Cell damage detection assay
Also known as:
- Spanish: Kit de citotoxicidad LDH, Ensayo de lactato deshidrogenasa, Ensayo de daño celular
- French: Kit de cytotoxicité LDH, Test de lactate déshydrogénase, Détection de dommages cellulaires
- German: LDH-Zytotoxizitätsassay, Lactatdehydrogenase-Test, Zellschädigungsassay
- Italian: Kit citotossicità LDH, Saggio lattato deidrogenasi, Rilevazione danno cellulare
FAQs
Q: What is measured in the LDH cytotoxicity assay?
A: LDH release, which indicates cell membrane damage. The assay is based on NADH-dependent reduction of a tetrazolium salt, generating a colorimetric signal.
Q: How long does the assay take?
A: Results are obtained within 30–60 minutes.
Q: Is it compatible with high-throughput assays?
A: Yes, optimized for 96- and 384-well plate formats.
Q: Can certain compounds interfere with the assay signal?
A: Yes, compounds affecting NADH-dependent or redox reactions may alter signal development in tetrazolium-based assays.