Magnesium Colorimetric Assay Kit

Magnesium is the 11th most abundant element in the human body. Mg²⁺ is essential for all living cells where it is involved in facilitating the processing of biological polyphosphates like DNA, RNA, ATP and enzyme functions. Mg²⁺ is the metallic ion at the center of chlorophyll. Mg²⁺ can be used as antacids and laxatives, and used to stabilize blood vessel spasm such as eclampsia and abnormal nerve excitation.

The Magnesium Colorimetric Assay Kit provides a simple and sensitive way for detection of magnesium concentration in various biological samples based on colorimetric method and the specific requirement of glycerol kinase for Mg²⁺. The enzyme linked reaction generates an intensely colored (λmax = 450 nm) product whose formation is proportional to Mg²⁺ concentration. The colored product can be easily qualified using a microtiter plate reader or a spectrophotometer at 450 nm. The linear range is 2-15 nmoles with detection sensitivity ~ 40 µM.