Size : 10g
Synonyms: Methylthionine chloride, Methylene Blue, Basic blue 9
| Description | Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM). |
| Targets & IC50 | Tau filament:1.9μM |
| Synonyms | Methylthionine chloride, Methylene Blue, Basic blue 9 |
| Cell Research | Instructions 1. Cell and tissue staining 1. Dissolve methylene blue in an appropriate solvent (such as water or buffer) to prepare a dye solution of a certain concentration. 2. Soak the cells or tissue sections in the dye solution, usually the staining time is several minutes. 3. After cleaning, observe the cell structure or distribution of target molecules under a microscope. 2. As MAO-A inhibitor and antidepressant and anti-anxiety drugs Methods: Through animal models or cell culture systems, the regulatory effect on neurotransmitters was studied. III. Research on neurodegenerative diseases Methods: Through animal models and cell models, its neuroprotective effects were studied. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 373.9 |
| Formula | C16H24ClN3O3S |
| Cas No. | 7220-79-3 |
| Smiles | O.O.O.[Cl-].CN(C)c1ccc2nc3ccc(cc3[s+]c2c1)N(C)C |
| Relative Density. | 0.98 g/cm3 at 25℃ |
| Storage | Store at low temperature,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||
| Solubility Information | DMSO: 3.75 mg/mL (10.03 mM), Sonication is recommended.  | ||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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