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> Mouse Anti-FLAG Tag monoclonal Antibody

Mouse Anti-FLAG Tag monoclonal Antibody

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Katalog-Nummer AM8495b-ev

Size : 50ul

Marke : Abcepta


FLAG Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

 
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  • WB - FLAG Tag Antibody AM8495b

    Anti-FLAG Tag Antibody at 1:2000 dilution + 12tag protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-FLAG Tag Antibody at 1:2000 dilution + 12tag protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Information

Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession unknow
Host Mouse
Clonality monoclonal
Isotype IgG1,κ
Clone/Animal Names 1557CT661.18.1

Additional Information

Target/Specificity KLH conjugated synthetic peptide encoding HA tag (DYKDDDDKC) was used as antigen.
Dilution WB~~1:2000
E~~Use at an assay dependent concentration.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFLAG Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information

Research Areas
  • Primary Antibodies
  • Crown Antibodies
Citations ( 0 )
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol

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