Mouse Anti-hnRNP K [3C2] - ChIP Grade
Katalog-Nummer MBS375183-0,1mg
Size : 0.1mg
Marke : MyBiosource
Anti-Hnrnp K (HNRNPK) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375183MBS375183 is an antibody targeting HNRNPK. Also known as Mouse Anti-hnRNP K [3C2] - ChIP Grade. Reactive in Mouse, Rat, Hamster, Cow, Human, Xenopus laevis. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
Anti-hnRNP K (HNRNPK), Monoclonal Antibody
Full Product Name
Mouse Anti-hnRNP K [3C2] - ChIP Grade
Product Gene Name
anti-HNRNPK antibody
Product Synonym Names
CSBP antibody; dC stretch binding protein antibody; FLJ41122 antibody; Heterogeneous nuclearribonucleoprotein K antibody; hnRNP K antibody; HNRNPK antibody; HNRPK antibody; HNRPK_HUMANantibody; Transformation up regulated nuclear antibody; Transformation up regulated nuclear proteinantibody; Transformation up-regulated nuclear protein antibody; Transformation upregulated nuclear proteinantibody; TUNP antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3C2
Host
Mouse
Species Reactivity
Mouse, Rat, Hamster, Cow, Human, Xenopus laevis
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide;
Purity/Purification
Protein A
Concentration
1mg/ml (lot specific)
Applications Tested/Suitable for anti-HNRNPK antibody
Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ChIP/Chip, Immunohistochemistry (IHC) (rat)
Application Notes for anti-HNRNPK antibody
Optimal antibody dilution should be determined by titration, however as a guideline try; WB: 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa) Flow Cyt: Use 1ug for 106 cells ChIP/Chip: Use at an assay dependent dilution. PubMed: 20673990
Product References and Citations for anti-HNRNPK antibody
Gao R et al. Heterogeneous nuclear ribonucleoprotein K (hnRNP-K) promotes tumor metastasis by induction of genes involved in extracellular matrix, cell movement, and angiogenesis.J Biol Chem. 2013 May 24;288(21):15046-56. doi: 10.1074/jbc.M113.466136. Epub 2013 Apr 5. ICC/IF; Human PMID: 23564449 Marchand V et al. Identification of protein partners of the human immunodeficiency virus 1 tat/rev exon 3 leads to the discovery of a new HIV-1 splicing regulator, protein hnRNP K.RNA Biol. 2011 Mar-Apr;8(2):325-42. Epub 2011 Mar 1.IP WB ; Human PMID: 21368586 Huarte M et al. A Large Intergenic Noncoding RNA Induced by p53 Mediates Global Gene Repression in the p53 Response. Cell 142:409-419 (2010). ChIP/Chip ; Mouse PMID: 20673990 Chang JW et al. hnRNP-K is a nuclear target of TCR-activated ERK and required for T-cell late activation.Int Immunol. 2009 Dec;21(12):1351-61. doi: 10.1093/intimm/dxp106. Epub 2009 Oct 30. WB ; Human PMID: 19880579 Enge M et al. MDM2-dependent downregulation of p21 and hnRNP K provides a switch between apoptosis and growth arrest induced by pharmacologically activated p53.Cancer Cell. 2009 Mar 3;15(3):171-83. doi: 10.1016/j.ccr.2009.01.019.WB ChIP ; Human PMID: 19249676 Fukuda T et al. hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP level during stress conditions.Genes Cells. 2009 Feb;14(2):113-28. doi: 10.1111/j.1365-2443.2008.01256.x. Epub 2008 Jan 6.ICC/IF WB ; Mouse IP WB ; Human PMID: 19170760 Schulz M et al. Proteomic analysis of peripheral blood mononuclear cells: selective protein processing observed in patients with rheumatoid arthritis.J Proteome Res. 2007 Sep;6(9):3752-9. Epub 2007 Aug 17.WB ; Human PMID: 17705417 Guil S et al. The multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a.Nat Struct Mol Biol. 2007 Jul;14(7):591-6. Epub 2007 Jun 10.WB ; Human PMID: 17558416 Inoue A et al. Loss-of-function screening by randomized intracellular antibodies: identification of hnRNP-K as a potential target for metastasis.Proc Natl Acad Sci U S A. 2007 May 22;104(21):8983-8. Epub 2007 May 4.WB ; Human PMID: 17483488 Cell-type-specific and developmental regulation of heterogeneous nuclear ribonucleoprotein K mRNA in the rat nervous system. Blanchette AR et al. Gene Expr Patterns. 2006 Aug;6(6):596-606. Epub 2006 Feb 20PMID: 16488668 Griffith BN et al. Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing.Biochim Biophys Acta. 2006 Nov-Dec;1759(11-12):552-61. Epub 2006 Oct 6.WB ; Mouse PMID: 17095106 Szeszel-Fedorowicz W et al. An exonic splicing silencer is involved in the regulated splicing of glucose 6-phosphate dehydrogenase mRNA.J Biol Chem. 2006 Nov 10;281(45):34146-58. Epub 2006 Sep 15.WB ; Human Rat PMID: 16980303 Blanchette AR et al. Cell-type-specific and developmental regulation of heterogeneous nuclear ribonucleoprotein K mRNA in the rat nervous system.Gene Expr Patterns. 2006 Aug;6(6):596-606. Epub 2006 Feb 20.IHC ; Rat PMID: 16488668 Yano M et al. Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation.J Biol Chem. 2005 Apr 1;280(13):12690-9. Epub 2005 Jan 25.WB ; Mouse PMID: 15671036 Characterization and primary structure of the poly(C)binding heterogeneous nuclear ribonucleoprotein complex K protein. Matunis M (Cell. Biol,Jan 1992)PMID: 1729596
Other Notes
Small volumes of anti-HNRNPK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for HNRNPK. It may not necessarily be applicable to this product.
NCBI Official Full Name
heterogeneous nuclear ribonucleoprotein complex K
NCBI Official Synonym Full Names
heterogeneous nuclear ribonucleoprotein K
NCBI Official Symbol
HNRNPK
NCBI Official Synonym Symbols
CSBP; TUNP; HNRPK
UniProt Protein Name
Heterogeneous nuclear ribonucleoprotein K
UniProt Synonym Protein Names
Transformation up-regulated nuclear protein; TUNP
Protein Family
Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name
HNRNPK
UniProt Synonym Gene Names
HNRPK; hnRNP K; TUNP
UniProt Entry Name
HNRPK_HUMAN
NCBI Protein Information
heterogeneous nuclear ribonucleoprotein K; dC-stretch binding protein; transformation upregulated nuclear protein
NCBI Summary for HNRNPK
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for HNRNPK
hnRNP K: a heterogeneous nuclear ribonucleoprotein (hnRNP). One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Two alternatively spliced isoforms have been described.
Protein type: Spliceosome; RNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 9q21.32-q21.33
Cellular Component: nucleoplasm; cell projection; focal adhesion; membrane; cytoplasm; nuclear chromatin; podosome; nucleus
Molecular Function: protein binding; RNA binding; single-stranded DNA binding
Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; viral reproduction; transcription, DNA-dependent; RNA splicing; positive regulation of low-density lipoprotein receptor biosynthetic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of receptor-mediated endocytosis
Show more Protein type: Spliceosome; RNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 9q21.32-q21.33
Cellular Component: nucleoplasm; cell projection; focal adhesion; membrane; cytoplasm; nuclear chromatin; podosome; nucleus
Molecular Function: protein binding; RNA binding; single-stranded DNA binding
Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; viral reproduction; transcription, DNA-dependent; RNA splicing; positive regulation of low-density lipoprotein receptor biosynthetic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of receptor-mediated endocytosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-HNRNPK antibody
| Reference Product | PubMed Publications |
|---|---|
| HNRNPA2B1 antibody | >3 publications with HNRNPK and HNRNPA2B1 |
| HNRNPL antibody | >2 publications with HNRNPK and HNRNPL |
| PTBP1 antibody | >1 publications with HNRNPK and PTBP1 |
| HNRNPA1 antibody | >1 publications with HNRNPK and HNRNPA1 |
| HNRNPF antibody | >1 publications with HNRNPK and HNRNPF |
Pathways associated with anti-HNRNPK antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Herpes Simplex Infection Pathway antibodies | Herpes Simplex Infection Pathway Diagram |
| Herpes Simplex Infection Pathway antibodies | Herpes Simplex Infection Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies | Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram |
| Spliceosome Pathway antibodies | Spliceosome Pathway Diagram |
| Spliceosome Pathway antibodies | Spliceosome Pathway Diagram |
| Viral Carcinogenesis Pathway antibodies | Viral Carcinogenesis Pathway Diagram |
Diseases associated with anti-HNRNPK antibody
| Disease Name | Pubmed Publications |
|---|---|
| Carcinoma Antibodies | >14 publications with HNRNPK and Carcinoma |
| Disease Models, Animal Antibodies | >3 publications with HNRNPK and Disease Models, Animal |
| Liver Neoplasms, Experimental Antibodies | >3 publications with HNRNPK and Liver Neoplasms, Experimental |
| Liver Neoplasms Antibodies | >3 publications with HNRNPK and Liver Neoplasms |
| Precancerous Conditions Antibodies | >3 publications with HNRNPK and Precancerous Conditions |
| Immune System Diseases Antibodies | >3 publications with HNRNPK and Immune System Diseases |
| Carcinoma, Hepatocellular Antibodies | >2 publications with HNRNPK and Carcinoma, Hepatocellular |
| Fibrosis Antibodies | >2 publications with HNRNPK and Fibrosis |
| Breast Neoplasms Antibodies | >2 publications with HNRNPK and Breast Neoplasms |
| Hypertrophy Antibodies | >1 publications with HNRNPK and Hypertrophy |
Organs/Tissues associated with anti-HNRNPK antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >9 publications with HNRNPK and Blood |
| Nerve Antibodies | >7 publications with HNRNPK and Nerve |
| Liver Antibodies | >6 publications with HNRNPK and Liver |
| Lung Antibodies | >4 publications with HNRNPK and Lung |
| Kidney Antibodies | >4 publications with HNRNPK and Kidney |
| Muscle Antibodies | >4 publications with HNRNPK and Muscle |
| Brain Antibodies | >4 publications with HNRNPK and Brain |
| Embryonic Tissue Antibodies | >3 publications with HNRNPK and Embryonic Tissue |
| Bone Antibodies | >3 publications with HNRNPK and Bone |
| Vascular Antibodies | >3 publications with HNRNPK and Vascular |