Mouse Anti-Mouse I-Ek-PE
Katalog-Nummer MBS673651-0,1mg
Size : 0.1mg
Marke : MyBiosource
Mouse Anti-Mouse I-Ek Secondary Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673651MBS673651 is a secondary antibody targeting Mouse Anti-Mouse I-Ek. Also known as Mouse Anti-Mouse I-Ek-PE. Reactive in Mouse. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
Mouse Anti-Mouse I-Ek, Secondary Antibody
Full Product Name
Mouse Anti-Mouse I-Ek-PE
Product Synonym Names
Mouse Anti-Mouse I-Ek - Phycoerythrin-Labeled; Mouse Anti-Mouse I-Ek
Clonality
Monoclonal
Isotype
Mouse (C3H.SW) IgG2a kappa
Clone Number
14-4-4S
Host
Mouse
Species Reactivity
Mouse
Form/Format
R - phycoerythrin (R - PE) Conjugate
Specificity
An epitope on mouse I-Ek MHC class II alloantigen
The monoclonal antibody 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes.1 Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2 The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D.3
The monoclonal antibody 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes.1 Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2 The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D.3
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Immunogen
C3H mouse skin graft and splenocytes
Characterization
To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent.
Applications Tested/Suitable for Mouse Anti-Mouse I-Ek secondary antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes for Mouse Anti-Mouse I-Ek secondary antibody
Research Application(s): In vitro induction of antigen-specific B cell differentiation
In vitro blocking of antigen presentation
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
In vitro blocking of antigen presentation
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Product References and Citations for Mouse Anti-Mouse I-Ek secondary antibody
1. Ozato, K., N. Mayer, and D.H. Sachs. 1980. J. Immunol. 124:533.
2. Klein, J. 1986. Mutations in H-2E loci. In: Natural History of the Major Histocompatibility Complex. John Wiley & Sons, New York, NY, pp 216-218.
3. Neiss, U., and K. Reske. 1994. Int. Immunol. 6:61.
4. Harton, J.A., W. Litaker, J.A. Frelinger, and G.A. Bishop. 1991. Immunogenetics 34:358.
5. Roy, M., A. Aruffo, J. Ledbetter, P. Linsley, M. Kehry, and R. Noelle. 1995. Eur. J. Immunol. 25:596.
6. Farr, A., P.C. DeRoos, S. Eastmaan, and A.Y. Rudensky. 1996. Eur. J. Immunol. 26:3185.
7. Pennesi, G., M.J., S. Sun, D. Avichezer, P.B. Silver, Z. Karabekian, C.S. David, P.A. Hargrave, J.H. McDowell, W.C. Smith, B. Wiggert, L.A. Donoso, C. Chan, and R.R. Caspi. 2003. J. Clin. Invest. 111:1171.
2. Klein, J. 1986. Mutations in H-2E loci. In: Natural History of the Major Histocompatibility Complex. John Wiley & Sons, New York, NY, pp 216-218.
3. Neiss, U., and K. Reske. 1994. Int. Immunol. 6:61.
4. Harton, J.A., W. Litaker, J.A. Frelinger, and G.A. Bishop. 1991. Immunogenetics 34:358.
5. Roy, M., A. Aruffo, J. Ledbetter, P. Linsley, M. Kehry, and R. Noelle. 1995. Eur. J. Immunol. 25:596.
6. Farr, A., P.C. DeRoos, S. Eastmaan, and A.Y. Rudensky. 1996. Eur. J. Immunol. 26:3185.
7. Pennesi, G., M.J., S. Sun, D. Avichezer, P.B. Silver, Z. Karabekian, C.S. David, P.A. Hargrave, J.H. McDowell, W.C. Smith, B. Wiggert, L.A. Donoso, C. Chan, and R.R. Caspi. 2003. J. Clin. Invest. 111:1171.
Other Notes
Small volumes of Mouse Anti-Mouse I-Ek secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.