Mouse CD11b/Mac-1a Antibody : APC/CY7
Katalog-Nummer OASB00333
Size : 0.1mg
Marke : Aviva Systems Biology
Mouse CD11b/Mac-1a Antibody : APC/CY7 (OASB00333)
| Datasheets/Manuals | Printable datasheet for Mouse CD11b/Mac-1a Antibody : APC/CY7 (OASB00333) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | M1/70 |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | APC-Cyanine 7 |
| Application | FC |
| Additional Information | Description: CD11b, the integrin alphaM subunit, combines with CD18, the integrin beta2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
| Concentration | 0.1 mg/mL |
| Specificity | CD11b |
| Characterization | To insure acceptable performance, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate Phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate Cyanine 5 conjugate R-phycoerythrin-Cyanine 7 conjugate Allophycocyanin-Cyanine 7 conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 1 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells <= 0.3 ug/106 cells |
| Application Info | Flow cytometry 1 In vitro and in vivo blocking 3, 5, 12 Immunohistochemistry (frozen sections) 11 (paraffin sections)13 Immunoprecipitation 2, 5 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 1 Ug/106 cells BALB/c splenocytes were double stained with rat antimouse CD11b-FITC and rat anti-mouse Ly-6G-PE. Lymphocytes were gated and analyzed by flow cytometry. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), and Allophycocyanin- Cyanine7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Jackson, K.A., T. Mi, M.A. Goodell. 1999. Proc. Natl. Acad. Sci. USA. 96:14482 2. Springer, T., G. Galfre, D.S. Secher, and C. Milstein. 1979. Eur. J. Immunol. 9:301 3. Springer, T.A., D. Davignon, M.- K. Ho, K. Kurzinger, E. Martz, and F. Sanchez- Madrid. 1982. Immunol. Rev. 68:171 4. Beller, D.I., T.A. Springer, R.D. Schreiber. 1982. J. Exp. Med. 156:1000 5. Sanchez- Madrid, F., P. Simon, S. Thompson, and T.A. Springer. 1983. J. Exp. Med. 158:586 6. Lub, M., Y. van Kooyk, and C.J. Figdor. 1996. J. Leukoc. Biol. 59:648 7. Kantor, A.B., A. M. Stall, S. Adams, L.A. Herzenberg, and L.A. Herzenberg. 1992. Proc. Natl. Acad. Sci. USA 89:3320 8. Ault, K.A., and T.A. Springer. 1981. J. Immunol. 126:359 9. Gresham, H.D., I.L. Graham, D.C. Anderson, and E.J. Brown. 1991. J. Clin. Invest. 88:588 10. Springer, T.A. 1994. Cell 76:310 11. Barbara, J.A., S.E. Turvey, S.I. Kingsley, B.M. Spriewald, M. Hara, O. Witzke, P.J. Morris, and K.J. Wood. 2000. Transplantation. 70:1641 12. Gyetko, M.R., S. Sud, T. Kendall, J.A. Fuller, M. W. Newstead, and T.J. Standiford. 2000. J. Immunol. 165:1513 13. Meyer, D., C. Schiller, J. Westermann, S. Izui, W.W. Hazenbos, J.S. Verbeek, R.E. Schmidt, and J.E. Gessner. 1998. Blood. 92:3997. |
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| Gene Symbol | ITGAM |
| Gene Full Name | Integrin alpha-M |
| Alias Symbols | CR, CR3, CR3A, Ly-4, MAC1, Mac-, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik |
| NCBI Gene Id | 16409; 3684 |
| Protein Name | integrin alpha-M |
| Uniprot ID | P05555, P11215 |
