Quantibody® Mouse Cytokine Array 1 Kit. Detects 20 Mouse Cytokines. Suitable for all liquid sample types.
Mouse Cytokine Array Q1
Mouse Cytokine Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
|
IL-12 p70
| IL-13
| IL-17A
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-9
| KC (CXCL1)
|
MCP-1 (CCL2)
| M-CSF
| RANTES (CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-CYT-1.gal)
Excel (QAM-CYT-1_Analysis_Tool.xls)
citations
TREM2hi resident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis
- Species:
- Mouse
- Sample Type:
- heart tissue
- Year:
- 2023
- Journal Name:
- Nature Metabolism
Nogo-B mediates endothelial oxidative stress and inflammation to promote coronary atherosclerosis in pressure-overloaded mouse hearts
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Redox biology
GV1001 Inhibits the Severity of the Ligature-Induced Periodontitis and the Vascular Lipid Deposition Associated with the Periodontitis in Mice
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Peptide immunization against the C-terminal of alpha-synuclein reduces locomotor activity in mice overexpressing alpha-synuclein
- Species:
- Mouse
- Sample Type:
- sera
- Year:
- 2023
- Journal Name:
- Plos One
Large yellow tea polysaccharide ameliorates obesity-associated metabolic syndrome by promoting M2 polarization of adipose tissue macrophage
- Species:
- Mouse
- Sample Type:
- adipose tissues
- Year:
- 2023
- Journal Name:
- Food & Function
Repeated intravenous administration of hiPSC-MSCs enhance the efficacy of cell-based therapy in tissue regeneration
- Species:
- Mouse
- Sample Type:
- limb tissue
- Year:
- 2022
- Journal Name:
- Communications biology
In Situ Growth of Tunable Gold Nanoparticles by Titania Nanotubes Templated Electrodeposition for Improving Osteogenesis through Modulating Macrophages Polarization
- Species:
- Mouse
- Sample Type:
- RAW264.7 cell
- Year:
- 2022
- Journal Name:
- Acs Applied Materials & Interfaces
Uncovering the Molecular Mechanism of the Qiang-Xin 1 Formula on Sepsis-Induced Cardiac Dysfunction Based on Systems Pharmacology
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2020
- Journal Name:
- Oxidative Medicine and Cellular Longevity
Lipoxin B4 promotes the resolution of allergic inflammation in the upper and lower airways of mice
- Species:
- Mouse
- Sample Type:
- Serum (Allergic Inflammation)
- Year:
- 2014
- Journal Name:
- Mucosal Immunology
- PubMed ID:
- 25465102
Nerve Growth Factor Regulates Neurolymphatic Remodeling during Corneal Inflammation and Resolution
- Species:
- Mouse
- Sample Type:
- Cell Lysate (Human Keratinocyte cell cultures); Tissue Lysate (Corneal Lysates)
- Year:
- 2014
- Journal Name:
- Plos One