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Mouse Monoclonal Anti-Tenascin C (Stromal Marker For Epithelial Malignancy) [Clone SPM319]
Katalog-Nummer NB-36-00304X-P1
Size : 100ug
Marke : Neo Biotech
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Telefonnummer : +1 850 650 7790
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Printable Datasheet
Tenascin C (Stromal Marker for Epithelial Malignancy) Antibody [SPM319]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified Tenascin C Mouse Monoclonal Antibody (SPM319). Confirmation of Integrity and Purity of Antibody.
Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Tenascin C Mouse Monoclonal Antibody (SPM319).
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cancer, Cardiovascular, Neuroscience, Angiogenesis
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
TNC
Positive Control
Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.
Immunogen
Human breast carcinoma
Alternate Names
Tenascin, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, Cytotactin; Glioma-associated-extracellular matrix antigen; GMEM; GP 150-225; Hexabrachion (HXB); JI; Myotendinous antigen; Neuronectin; TNC
Functions
- Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327).
Key References
- Verstraeten AA, et. al. British Journal of Dermatology, 1992, 127(6):571-4.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/1704365/
- https://pubmed.ncbi.nlm.nih.gov/1707164/
- https://pubmed.ncbi.nlm.nih.gov/1719530/
- https://pubmed.ncbi.nlm.nih.gov/7531707/
- https://pubmed.ncbi.nlm.nih.gov/15164053/
- https://pubmed.ncbi.nlm.nih.gov/7524681/
- https://pubmed.ncbi.nlm.nih.gov/2466295/
- https://pubmed.ncbi.nlm.nih.gov/8824254/
- https://pubmed.ncbi.nlm.nih.gov/16335952/
- https://pubmed.ncbi.nlm.nih.gov/18780401/
- https://pubmed.ncbi.nlm.nih.gov/19159218/
- https://pubmed.ncbi.nlm.nih.gov/19884327/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
- https://pubmed.ncbi.nlm.nih.gov/24275569/
- https://pubmed.ncbi.nlm.nih.gov/26091039/
- https://pubmed.ncbi.nlm.nih.gov/1279805/
- https://pubmed.ncbi.nlm.nih.gov/23936043/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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