Mouse monoclonal antibody to Cytokeratin 18
Katalog-Nummer CPA9148-100ul
Size : 100ul
Marke : Cohesion Biosciences
Contact local distributor :
Telefonnummer : +1 850 650 7790
- Western blot analysis of Cytokeratin 18 expression in HepG2 (A), Hela (B), mouse liver (C), mouse skeletal muscle (D), C2C12 (E), rat heart (F) whole cell lysates.
- Immunofluorescent analysis of Cytokeratin 18 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Product Name | Anti-Cytokeratin 18 Antibody |
| Cat No: | CPA9148 |
| Source: | Mouse |
| Reactivity: | H, M, R |
| Applications: | WB, IF/IC |
| *Application Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
| *Species Reactivity Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
Description: Mouse monoclonal antibody to Cytokeratin 18 Immunogen: Recombinant protein corresponding to human Cytokeratin 18. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200) Gene Symbol: KRT18 Alternative Names: CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18Entrez Gene (Human): Entrez Gene (Mouse) : Entrez Gene (Rat) : SwissProt (Human): SwissProt (Mouse) : SwissProt (Rat) : Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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