N-Cadherin antibody

Katalog-Nummer GTX127345-100ul

Size : 100ul

Marke : GeneTex


N-Cadherin antibody

Cat. No. GTX127345
3 99
Cat. No.  GTX127345
KO/KD Orthogonal
Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345
GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image
KO/KD

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image
Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX127345
GTX127345 IHC-P Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
N-Cadherin stained by N-Cadherin antibody (GTX127345) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345
GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody (GTX127345) diluted at 1:500.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127345
GTX127345 ICC/IF Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image
Orthogonal

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
GTX127345 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

This N-Cadherin antibody (GTX127345) is a rabbit polyclonal antibody validated for detection of N-Cadherin by WB, ICC/IF, IHC-P, and IHC-Fr. Reactive to human, mouse, rat, and other species. This product is cited in 99 published studies. Knockout/knockdown validation supports antibody specificity.
Summary
N-Cadherin antibody recognizes N-cadherin protein, a calcium-dependent cell adhesion protein (predicted molecular weight of 100 kDa) encoded by the CDH2 gene. N-cadherin is involved in multiple processes, including neuron development and epithelial-mesenchymal transition (EMT). Due to the prominent role of EMT in cancer progression and metastasis, antibodies targeting either N-cadherin or E-cadherin are frequently used together to study the EMT process: the upregulation of N-cadherin followed by the downregulation of E-cadherin.
See all N-Cadherin products

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