NeoLume™ ECL Tablet Substrate – Ultra Sensitivity
Description
NeoLume ECL Tablet Substrate from Neo Biotech is a next-generation chemiluminescent substrate for researchers requiring exceptional sensitivity, high signal quality, and a more sustainable approach to Western blot detection. It combines low-femtogram detection, high signal intensity, and a single-use tablet format for demanding protein analysis applications.
Each experiment requires one tablet of Formulation A and one tablet of Formulation B, dissolved together in 5 mL of purified water, providing enough substrate for a 50 cm2 membrane. Dissolution takes less than 15 minutes and can be performed during secondary antibody incubation.
Direct alternatives: SuperSignal™ West Femto (Thermo Scientific™), Clarity Max™ (Bio-Rad), and Amersham™ ECL Select™ (Cytiva), without changes to existing blocking conditions or antibody concentrations.
Key Performance Data at a Glance
| Metric | Performance |
|---|---|
| Detection level | Low-femtogram. |
| Format | Patented single-use tablets: Formulation A + Formulation B. |
| Signal-to-noise ratio | Exceptional, with very low background. |
| Preparation time | Less than 15 minutes; can overlap with secondary antibody incubation. |
| Membrane coverage per reaction | 50 cm2: one tablet A + one tablet B in 5 mL of purified water. |
| Shelf life | 1 year at 2–8 °C. |
| Shipping | Room temperature (15–25 °C). |
Why Choose NeoLume ECL Tablet Substrate?
Outstanding Sensitivity for Challenging Western Blots
Designed for extremely low-abundance proteins, NeoLume ECL Tablet Substrate produces a strong, stable chemiluminescent signal with low background. Its high signal-to-noise ratio supports clear visualization of weak targets in demanding research applications.
Seamless Replacement for Premium ECL Substrates
It is a direct alternative to SuperSignal™ West Femto, Clarity Max™, and Amersham™ ECL Select™. Researchers can adopt the substrate without changing existing Western blot protocols, including blocking conditions and antibody concentrations.
Innovative Single-Use Tablet Format
Preparation takes less than 15 minutes and can be performed during secondary antibody incubation. The compact, lightweight format reduces contamination risk, enables compact storage, and supports more sustainable use of water and materials.
High Performance with Reduced Antibody Consumption
Its exceptional sensitivity supports highly diluted antibodies while maintaining excellent detection performance, helping laboratories reduce reagent costs without compromising analytical sensitivity or data quality.
Product Properties
| Property | Details |
|---|---|
| Description | Ultra-sensitive ECL substrate with strong signal intensity, low background, and stable light output at the low-femtogram level, supplied in a sustainable single-use tablet format. The standard kit provides 20 single uses. For each use, dissolve one Formulation A tablet and one Formulation B tablet together in 5 mL of purified water, sufficient for a 50 cm2 membrane. Dissolution takes less than 15 minutes and can be performed during secondary antibody incubation. |
| Features | Compact and lightweight, with sustainable water use and reduced contamination risk. Innovative single-use tablet format for ECL Western blotting. Replaces leading high-performance substrates without protocol changes. |
| Components | Luminol/enhancer tablets (A). Peroxide tablets (B). |
| Antibody dilution range | Primary: 1:5,000–1:100,000. Secondary: 1:100,000–1:500,000. (From 1 mg/mL stock solutions.) |
| Storage | Stable for 1 year at 2–8 °C. |
| Shipping | Room temperature (15–25 °C). |