Description

• NeoSupreme qPCR Green Master Mix (2x), ROX plus: optimized for sensitive, fast, and reproducible qPCR.
• Hot-start enzyme mechanism enhances detection sensitivity and specificity.
• Contains green intercalating dye, dNTPs, stabilizers, enhancers, and ROX plus reference dye.
• Designed for accurate gene expression analysis and broad instrument compatibility.
• Ready to use: simply add primers and DNA template.

Storage Conditions

Upon arrival, all components should be stored at -85 °C to -15 °C in a constant temperature freezer to guarantee maximal shelf life. Minimize the number of freeze-thaw cycles by storing it in working aliquots. The master mix remains stable at 2-8 °C for up to 5 months. Do not expose the master mix to direct sunlight. The product will remain stable till the expiry date if stored as specified.

Compatibility with real-time PCR instruments

NeoSupreme qPCR Green Master Mix (2x), ROX plus is compatible with instruments that measure the passive reference signal. However, it is also compatible with instruments that do not require a passive reference signal for data normalization. It was optimized to be compatible with the following real-time PCR instruments:

Applied Biosystems: 7000; 7300; 7700; 7900; 7900HT; 7900HT FAST; StepOne™ & StepOne™plus

Quality control assays

Genomic DNA contamination

The product must comply with internal standards of DNA contamination as evaluated through real-time PCR.

Nuclease assays

To test for DNase contamination, 0.2-0.3 μg of plasmid DNA are incubated with the master mix for 14-16 h at 37 °C. To test for RNase contamination, 1 µg of RNA is incubated with the master mix for 1 h at 37 °C. Following incubation, the nucleic acids are visualized on a GreenSafe-stained agarose gel. There must be no visible nicking or cutting of the nucleic acids.

Functional assay

NeoSupreme qPCR Green Master Mix (2x), ROX plus is extensively tested for activity, processivity, efficiency, sensitivity and heat activation.