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> Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Powder)

Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Powder)

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Katalog-Nummer MBS652750-100L

Size : 100L

Marke : MyBiosource


Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652750

Ham's Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation,… More details

Product Overview

Product Name
Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate, Culture Media
Full Product Name
Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Powder)
Purity/Purification
Cell Culture Grade
Preparation and Storage
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.
Solubility
Light pink, clear, complete
pH
7.4
Endotoxin
≤ 1EU/ml
Appearance
Off-white, homogeneous, free flowing powder
Additional Note
Hygroscopic. Keep tightly closed and dessicated.
Directions per Liter
Dissolve 11.75g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as g/liter
Inorganic salts:
Calcium Chloride•2H2O: 0.165
Cupric Sulfate•5H2O: 0.0000025
Ferrous Sulfate•7H2O: 0.000834
Magnesium Chloride•6H2O: 0.09963
Magnesium Sulfate: 0.02528
Potassium Chloride: 0.305
Potassium Phosphate Mono: 0.06124
Sodium Chloride: 7.517
Sodium Phosphate Dibasic: 0.1324
Zinc Sulfate•7H2O: 0.000863

Amino Acids:
L-Alanine: 0.018
L-Arginine•HCl: 0.422
L-Asparagine•H2O: 0.03
L-Aspartic Acid: 0.026
L-Cysteine•HCL•H2O: 0.07026
L-Glutamic Acid: 0.03
L-Glutamine: 0.292
Glycine: 0.016
L-Histidine•HCl•H2O: 0.042
L-Isoleucine: 0.0078
L-Leucine: 0.0262
L-Lycine•HCl: 0.073
L-Methionine: 0.009
L-Phenylalanine: 0.01
L-Proline: 0.07
L-Serine: 0.021
L-Threonine: 0.0238
L-Tryptophan: 0.004
L-Tyrosine•2Na•2H2O: 0.01586
L-Valine: 0.0234

Vitamins:
L-Ascorbic Acid•Na: 0.15
D-Biotin: 0.0000073
Choline Chloride: 0.01396
Vitamin B-12: 0.00136
Folic Acid: 0.00132
myo-Inositol: 0.01802
Niacinamide: 0.00004
D-Pantothenic Acid, Ca: 0.000238
Pyridoxine•HCl: 0.00006
Riboflavin: 0.00004
Thiamine•HCl: 0.000337

Other:
Thymidine: 0.0007
D-Glucose: 1.802
Hypoxanthine: 0.00404
Linoleic Acid: 0.00009
Thioctic Acid: 0.000206
Phenol Red, Sodium: 0.00125
Putrescine•2HCl: 0.000161
Pyruvic Acid, Sodium: 0.22

Total: 11.75 g/liter

Product Categories/Family for Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate culture media

Culture Media; Media-Cell Culture; Nutrient Mixture Ham's

Other Notes

Small volumes of Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate purchase

MBS652750 is a culture media targeting Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate. Also known as Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Powder).

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