Phospho-MLK3 (Thr277+Ser281) Antibody
Katalog-Nummer MBS9614080-0,1mL
Size : 0.1mL
Marke : MyBiosource
Western blot analysis of extracts from mouse brain, using MLK3 (Phospho-Thr277+Ser281) Antibody.
Western blot analysis of MLK3 (Phospho-Thr277+Ser281) using K562 whole cell lysates.
-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).
-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).
At 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
At 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
MLK3 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9614080MBS9614080 is an antibody targeting MLK3. Also known as Phospho-MLK3 (Thr277+Ser281) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details
Product Overview
Online Datasheet in PDFProduct Name
MLK3, Polyclonal Antibody
Full Product Name
Phospho-MLK3 (Thr277+Ser281) Antibody
Product Gene Name
anti-MLK3 antibody
Product Synonym Gene Name
MAP3K11
Product Synonym Names
2610017K16Rik; EC 2.7.11.25; M3K11_HUMAN; Map3k11; MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1; PTK1; RHOE; SH3 domain containing proline rich kinase; SPRK; Src-homology 3 domain-containing proline-rich kinase
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Peptide affinity purification
Concentration
~1mg/ml (lot specific)
Specificity
Phospho-MLK3 (Thr277+Ser281) Antibody detects endogenous levels of MLK3 only when phosphorylated at Thr277+Ser281.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
MLK3 (Thr277+Ser281) peptide (MBS9619042) is used for blocking the activity of MLK3 (Thr277+Ser281) antibody (MBS9614080)
Molecular Weight
92KD
OMIM
600050
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human MLK3 around the phosphorylation site of Thr277+Ser281.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MLK3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes for anti-MLK3 antibody
WB: 1:1000-3000
IHC: 1:50-1:200
ELISA (peptide): 1:20,000-1:40,000
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
ELISA (peptide): 1:20,000-1:40,000
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (IHC) of anti-MLK3 antibody
At 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
Immunohistochemistry (IHC) of anti-MLK3 antibody
At 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
Other Notes
Small volumes of anti-MLK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for MLK3. It may not necessarily be applicable to this product.
NCBI Official Full Name
Mitogen-activated protein kinase kinase kinase 11
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 11
NCBI Official Symbol
MAP3K11
NCBI Official Synonym Symbols
MLK3; PTK1; SPRK; MLK-3; MEKK11
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 11
UniProt Synonym Protein Names
Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase
UniProt Gene Name
MAP3K11
UniProt Entry Name
M3K11_HUMAN
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 11
NCBI Summary for MLK3
The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for MLK3
MLK3: a TKL kinase of the MLK family. Has an amino-terminal SH3 domain followed by the kinase domain, two leucine zipper domains, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. Phosphorylates IkappaB and activates SEK1 and MKK7.
Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily
Chromosomal Location of Human Ortholog: 11q13.1-q13.3
Cellular Component: centrosome; cytoplasm; membrane; microtubule
Molecular Function: ATP binding; identical protein binding; JUN kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity; Rac GTPase binding
Biological Process: activation of JNK activity; activation of JNKK activity; activation of MAPK activity; cell death; cell proliferation; JNK cascade; microtubule-based process; peptidyl-tyrosine phosphorylation; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of neuron apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation
Show more Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily
Chromosomal Location of Human Ortholog: 11q13.1-q13.3
Cellular Component: centrosome; cytoplasm; membrane; microtubule
Molecular Function: ATP binding; identical protein binding; JUN kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity; Rac GTPase binding
Biological Process: activation of JNK activity; activation of JNKK activity; activation of MAPK activity; cell death; cell proliferation; JNK cascade; microtubule-based process; peptidyl-tyrosine phosphorylation; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of neuron apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-MLK3 antibody
| Reference Product | PubMed Publications |
|---|---|
| NF2 antibody | >1 publications with MLK3 and NF2 |
Pathways associated with anti-MLK3 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| ARMS-mediated Activation Pathway antibodies | ARMS-mediated Activation Pathway Diagram |
| Axon Guidance Pathway antibodies | Axon Guidance Pathway Diagram |
| CDC42 Signaling Events Pathway antibodies | CDC42 Signaling Events Pathway Diagram |
| Cytokine Signaling In Immune System Pathway antibodies | Cytokine Signaling In Immune System Pathway Diagram |
| DAP12 Interactions Pathway antibodies | DAP12 Interactions Pathway Diagram |
| DAP12 Signaling Pathway antibodies | DAP12 Signaling Pathway Diagram |
| Developmental Biology Pathway antibodies | Developmental Biology Pathway Diagram |
| Downstream Signal Transduction Pathway antibodies | Downstream Signal Transduction Pathway Diagram |
| Downstream Signaling Of Activated FGFR1 Pathway antibodies | Downstream Signaling Of Activated FGFR1 Pathway Diagram |
| Downstream Signaling Of Activated FGFR2 Pathway antibodies | Downstream Signaling Of Activated FGFR2 Pathway Diagram |
Diseases associated with anti-MLK3 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Cell Transformation, Neoplastic Antibodies | >1 publications with MLK3 and Cell Transformation, Neoplastic |
| Prostatic Diseases Antibodies | >1 publications with MLK3 and Prostatic Diseases |
| Breast Neoplasms Antibodies | >1 publications with MLK3 and Breast Neoplasms |
| Inflammation Antibodies | >1 publications with MLK3 and Inflammation |
Organs/Tissues associated with anti-MLK3 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Testis Antibodies | >3 publications with MLK3 and Testis |
| Bone Marrow Antibodies | >1 publications with MLK3 and Bone Marrow |
| Blood Antibodies | >1 publications with MLK3 and Blood |
| Adrenal Gland Antibodies | >1 publications with MLK3 and Adrenal Gland |
| Uterus Antibodies | >1 publications with MLK3 and Uterus |
| Prostate Antibodies | >1 publications with MLK3 and Prostate |
| Brain Antibodies | >1 publications with MLK3 and Brain |
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