Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa)
Katalog-Nummer MBS355493-0,1mL
Size : 0.1mL
Marke : MyBiosource
Molecular weight range – 10-250 kDa.
Brightness reference band-70 kDa, 25 kDa.
Ready to use-Pre-mixed with loading buffer.
Narrow band.
Brightness reference band-70 kDa, 25 kDa.
Ready to use-Pre-mixed with loading buffer.
Narrow band.
Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) Accessory Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS355493Prestained protein ladder / marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and approximate sizing of proteins. The… More details
Product Overview
Online Datasheet in PDFProduct Name
Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa), Accessory Reagent
Full Product Name
Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa)
Form/Format
62.5mM Tris-H3PO4 (pH 7.5, 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Preparation and Storage
Store at 4 degree C for 3 months, and at -20 degree C for long term.
Features
• Molecular weight range - 10-250 kDa
• Brightness reference band -70 kDa, 25kDa
• Ready to use - Pre-mixed with loading buffer.
• Narrow band
• Brightness reference band -70 kDa, 25kDa
• Ready to use - Pre-mixed with loading buffer.
• Narrow band
Protocol
1: Thaw the ladder either at room tempeture or at 37°C for a few minutes to disolve precipitated solids thoroughly, Do not boil!
2: Mix gently, but thoroughly to ensure that the solution is homogenous.
3: Load the following volumes of the ladder on Min SDS-polyacrylamide gel: 5 ul per well, with a thickness of 0.75-1.0 mm.
Large SDS-polyacrylamide gel: 10 ul per well, with thickness of 0.75-1.0 mm.
2: Mix gently, but thoroughly to ensure that the solution is homogenous.
3: Load the following volumes of the ladder on Min SDS-polyacrylamide gel: 5 ul per well, with a thickness of 0.75-1.0 mm.
Large SDS-polyacrylamide gel: 10 ul per well, with thickness of 0.75-1.0 mm.
Product Categories/Family for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) accessory reagent
Molecular Weight Marker
Applications Tested/Suitable for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) accessory reagent
SDS-PAGE; Western Blot (WB)
Application Notes for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) accessory reagent
Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
Verifying Western transfer efficiency.
Verifying Western transfer efficiency.
Other Notes
Small volumes of Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) purchase
MBS355493 is an accessory reagent targeting Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa).