Rabbit IgM (mu) Antibody Peroxidase Conjugated

Katalog-Nummer orb346690-20mg

Size : 20mg

Marke : Biorbyt


Rabbit IgM (mu) Antibody Peroxidase Conjugated

SKU: orb346690

Description

Rabbit IgM (mu) antibody (Peroxidase)

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
ReactivityRabbit
Application Notes
Rabbit IgM (mu) peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of Rabbit IgM in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-Rabbit IgM (mu) was produced by repeated immunization with rabbit IgM mu heavy chain in goat.
PurityThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgM and Rabbit Serum. No reaction was observed against Rabbit IgG.
ConjugationHRP

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration10.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Rabbit IgM (mu) Antibody, Gt-a-Rabbit IgM (mu) Peroxidase conjugated, Rabbit IgM (mu) Peroxidase Antibody in goat, Goat peroxidase conjugated Secondary Antibody, goat anti-rabbit IgM (mu chain) HRP conjugated antibody

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol