Rabbit Mouse IgE (Fc specific) Antibody

Purified IgG fraction of polyclonal rabbit antiserum to mouse IgE, Fc specific. As unlabelled secondary antibody for indirect detection of IgE in mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immuno- conjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.