Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Anti-GRP78 Antibody

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Katalog-Nummer CPA7529-100ul

Size : 100ul

Marke : Cohesion Biosciences

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Western blot analysis of GRP78 expression in NIH3T3 (A) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 78 kD)
Immunohistochemical analysis of GRP78 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Name:	Anti-GRP78 Antibody
Cat No:	CPA7529
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to GRP78

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GRP78. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene Symbol：HSPA5

Alternative Names：GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

Entrez Gene (Human)： 3309;

SwissProt (Human)： P11021;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.