RayBio® G-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.
Rat Cytokine Array G2
Rat Cytokine Array G2
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Rat |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 34 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Activin A
| Agrin
| CD86 (B7-2)
| beta-NGF
| CINC-1 (CXCL1)
|
CINC-2
| CINC-3
| CNTF
| Fas Ligand (TNFSF6)
| Fractalkine (CX3CL1)
|
GM-CSF
| ICAM-1 (CD54)
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-1 R6 (IL-1 Rrp2)
| IL-2
| IL-4
| IL-6
| IL-10
|
IL-13
| Leptin
| LIX
| L-Selectin (CD62L)
| MCP-1 (CCL2)
|
MIP-3 alpha (CCL20)
| MMP-8
| PDGF-AA
| Prolactin R
| RAGE
|
TCK-1 (CXCL7)
| TIMP-1
| TNF alpha
| VEGF-A
| |
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- Rat Cytokine Antibody Array G2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAR-CYT-G2-4.gal)
GAL (GAL-AAR-CYT-G2-8.gal)
Excel (AAR-CYT-G2_Analysis_Tool.xls)
citations
Role of Serum Inflammatory Cytokines in Sepsis Rats Following BMSCs Transplantation: Protein Microarray Analysis
- Species:
- Rat
- Sample Type:
- Bone marrow stromal cells (BMSCs)
- Year:
- 2023
- Journal Name:
- Cell Transplantation
The role of HMGB1 in BMSC transplantation for treating MODS in rats
- Species:
- Rat
- Sample Type:
- Tissue Lysate
- Year:
- 2018
- Journal Name:
- Cell and Tissue Research
Mesenchymal stem cells attenuate peritoneal injury through secretion of TSG-6
- Species:
- Rat
- Sample Type:
- Conditioned Media (Mesechymal stem cells: Peritoneal Injury model, serum starved)
- Year:
- 2017
- Journal Name:
- Plos One
Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer's Disease-Like Amyloid- Pathology and Neuroinflammation in the Rat Hippocampus
- Species:
- Rat
- Sample Type:
- Cell Lysate (Rat Hippocampal tissue lysate)
- Year:
- 2015
- Journal Name:
- Scientific reports
Multiplex array proteomics detects increased MMP-8 in CSF after spinal cord injury
- Species:
- Rat
- Sample Type:
- CSF (CSF)
- Year:
- 2012
- Journal Name:
- Journal of Neuroinflammation
- PubMed ID:
- 22687332