AP05378FC-N | Rat IgG1 Goat Polyclonal Antibody Clinisciences

Rat IgG1 Goat Polyclonal Antibody

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SKU

AP05378FC-N

Rat IgG1 goat polyclonal antibody, FITC

<strong>Published customer image:</strong> <br>Horseradish peroxidase conjugated Goat anti Rat IgG1 antibody (<strong>STAR112P</strong>) used to evaluate post challenge antibody response in rats by ELISA.<br><strong>Image caption:</strong><br>Post-challenge serum antibody isotype responses of vaccinated and control rats to recombinant cysteine proteinase.<br>(A) IgG1. (B) IgM. (C) IgA. (D) IgE. Group G–Lyophilised lettuce expressing the mature CPFhW enzyme flanked by Gly-rich linkers; Group U–Lyophilised lettuce expressing the mature CPFhW protein fused with ubiquitin; Group C–Lyophilised control lettuce; Group N–None. At each timepoint, four rats from each group were euthanised and dissected. *Indicates significantly increased numbers of cells (p<0.05). Error bars indicate standard deviation.<br><br>From: Citation: Kesik-Brodacka M, Lipiec A, Kozak Ljunggren M, Jedlina L, Miedzinska K, Mikolajczak M, <em>et al</em>. (2017)<br>Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with <em>Fasciola hepatica</em> metacercariae.<br><a href="https://www.ncbi.nlm.nih.gov/pubmed/28333957"target="_blank">PLoS Negl Trop Dis 11(3): e0005451</a>.

Specifications
Product Data
Application	FC
Recommended Dilution	Flow Cytometry: Use 50 µl of 1/400 diluted antibody to label 1 x 10⁶ cells in 100 µl.
Reactivity	Rat
Antibody Host	Goat
Isotype	IgG
Clonality	Polyclonal
Specificity	This antibody recognises Rat gG1. The antibody has been shown to react specifically with Rat IgG1 by immunoelectrophoresis and ELISA. The antibody may cross react with IgG1 from other species.
Buffer	Phosphate buffered saline pH 7.2 Label: FITC State: Liquid purified IgG fraction Stabilizer: 0.2% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	FITC
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shipping	Blue Ice

Product Manuals
Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
SDS for AP05378FC-N

Antibody Resources
Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Rat IgG1 Goat Polyclonal Antibody

Katalog-Nummer AP05378FC-N

Rat IgG1 Goat Polyclonal Antibody

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Citations