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> Rat IgM (mu chain) antibody (Alkaline Phosphatase)

Rat IgM (mu chain) antibody (Alkaline Phosphatase)

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Katalog-Nummer orb347866-1mg

Size : 1mg

Marke : Biorbyt

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    • Rat IgM (mu chain) antibody (Alkaline Phosphatase)

    Rat IgM (mu chain) antibody (Alkaline Phosphatase)

    Catalog Number: orb347866

    Catalog Numberorb347866
    CategoryAntibodies
    DescriptionRat IgM (mu chain) antibody (Alkaline Phosphatase)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRat
    IsotypeIgG
    ImmunogenRat IgM whole molecule
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
    Form/AppearanceLiquid (sterile filtered)
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgM, and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
    ConjugationAP
    StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesRabbit Anti-Rat IgM (mu chain) Antibody alkaline p
    NoteFor research use only
    Application notesAnti-Rat IgM Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 µg of Rat IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
    Expiration Date12 months from date of receipt.
    Rat IgM (mu chain) antibody (Alkaline Phosphatase)

    Western Blot of Anti-Rat IgM (mu chain) (RABBIT) Antibody (p/n orb347851). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody (p/n orb347851) 1:2000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.