Recombinant human Chorionic Gonadotropin, Beta Polypeptid/hCG-beta overexpression cell culture supernatant, secreted from the serum-free medium of transfected HEK293 cells (200 µl). Purchase will also include one vial of normal control, the culture supernatant of HEK293 cell transfected with empty expression vector.
This protein is used as the standard in the ELISA kit (catalog number: ELH-HCGB).
Recombinant Human hCG-beta Protein, HEK293 Supernatant
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Product Description
Specifications
| Size | 200 µL |
| Species | Human |
| Accession Number | P01233 |
| Gene Symbols | CGB |
| Protein Name / Synonyms | Chorionic Gonadotropin, Beta Polypeptide, Chorionic Gonadotrophin Chain Beta, CGB3, Chorionic Gonadotropin Beta 3 Subunit, Chorionic Gonadotropin Beta Subunit, Chorionic Gonadotropin Beta Chain, Choriogonadotropin Subunit Beta, CGB CGB5 CGB7 CGB8, CGB5, CGB7, CGB8, HCGB, Choriogonadotropin subunit beta, CG-beta |
| Expressed Region | Ser21-Gln165 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | Recombinant protein has a calculated molecular mass of 16. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs). |
| Format | Pink liquid |
| Formulation | Culture supernatant of transfected HEK293 cells |
| Preservative | None |
| Endotoxin Level | Not determined |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.