Recombinant Human Nucleobindin-2
Katalog-Nummer CR25-500ug
Size : 500ug
Marke : Novoprotein
Description Recombinant Human Nucleobindin-2 is produced by our E.coli expression system and the target gene encoding Val25-Leu106 is expressed.
Accession P80303
Known As Nucleobindin-2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin; Nesfatin-1; NUCB2; NEFA
Mol Mass 9.6 KDa
APMol Mass 10 KDa, reducing conditions
Formulation Description Lyophilized from a 0.2 μm filtered solution of 10mM Sodium Phosphate, pH 6.5.
Endotoxin < 1 EU/µg as determined by LAL test.
Purity-SDS-PAGE 
Greater than 95% as determined by reducing SDS-PAGE. (QC verified) Purity-SEC-HPLC 
Greater than 95% as determined by SEC-HPLC. (Regularly tested) Reconstitution - Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping - The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
Storage - Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Background Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2). Nesfatin-1 is a neuropeptide produced in the hypothalamus of mammals. It participates in the regulation of hunger and fat storage. Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets β-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Nesfatin-1 may also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.