Recombinant Porcine Prolactin Protein

Katalog-Nummer 230-31006-50

Size : 50ug

Marke : RayBiotech


Recombinant Porcine Prolactin Protein

Recombinant porcine prolactin protein with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description

Specifications

Size50 µg
SpeciesPorcine (pig)
Accession Number
P01238
Gene Symbols
PRL
Protein Name / Synonyms
Decidual Prolactin, Growth Hormone A1, GHA1.
Expressed Region
Leu31-Cys229
Expression SystemHEK293 cells
TagC-terminal His-tag
Purity
>90%
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it also presented as 30 kDa extra protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format
Liquid
Formulation
Supplied as a 0.2 µm filtered solution in 1x PBS (pH 7.4) with 1% mannitol and 5% trehalose
Concentration (lot specific)
Refer to the label on the vial for the specific concentration, determined by the Pierce BCA Protein Assay Kit.
Endotoxin Level< 0.5 EU per μg of the protein as determined by the LAL method
Research AreaCytoskeletal Reorganization
Shipping TypeBlue ice
Storage-20°C
SDS-PAGE Image
Figure 1. Deglycosylation analysis of purified recombinant proteins. Product was either untreated (Lane 2) or treated with deglycosylation enzymes under native (Lane 3) or denaturing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift, indicating that the untreated protein (Lane 2) was glycosylated. Lane 1, protein ladder (kDa).
Storage/Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long-term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Generally, the shelf life is up to 6 months from the date of receipt at -20°C or -80°C under sterile condition.