STAT3 Leeporter Luciferase Reporter-NIH 3T3 Cell Line
Katalog-Nummer MBS669570-1
Size : 1Vial
Marke : MyBiosource
STAT3 Leeporter Luciferase Reporter-NIH 3T3 Cell Line
Fig-1: Induction of STAT3 activity by mIFN-gamma in STAT3 Leeporter™ – NIH 3T3 cells.
Fig-2: Induction of STAT3 activity by IL-6 in STAT3 Leeporter™ – NIH 3T3 cells.
STAT3 Cell Line
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS669570The STAT3 Leeporter Luciferase Reporter cell line is a stably transfected NIH 3T3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT3 responsive promoter, so that the cell line is designed to measure the… More details
Product Overview
Online Datasheet in PDFProduct Name
STAT3, Cell Line
Full Product Name
STAT3 Leeporter Luciferase Reporter-NIH 3T3 Cell Line
Product Gene Name
STAT3 cell line
Host
NIH 3T3 cells
Form/Format
Each vial contains 2-3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Preparation and Storage
Immediately upon receipt, store in liquid nitrogen.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Culture Conditions
Cells should be grown at 37 degree C with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3 ug/ml of Puromycin (Note: Puromycin can be omitted during the reporter cell assays). It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C-CO2 incubator. Leave the T25 flask in the incubator for 1~3 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are between 80~90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin.
Note: NIH 3T3 cells should be split before they reach 90% confluence; otherwise, they become self-lifted and aggregate irrevisibly. Precoating the cell plates with 0.1% gelatin may prevent NIH 3T3 cells from self-lifting. During cell trypsinization, cells covered enough with trypsin-EDTA solution should be stayed at 37 degree C for 10 min without agitation. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.
Note: NIH 3T3 cells should be split before they reach 90% confluence; otherwise, they become self-lifted and aggregate irrevisibly. Precoating the cell plates with 0.1% gelatin may prevent NIH 3T3 cells from self-lifting. During cell trypsinization, cells covered enough with trypsin-EDTA solution should be stayed at 37 degree C for 10 min without agitation. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Product Categories/Family for STAT3 cell line
Reporter Cell Lines
Applications Tested/Suitable for STAT3 cell line
Functional Assay (FA)
Application Notes for STAT3 cell line
Monitor the STAT3 signaling pathway activity. Screen for activators or inhibitors of the STAT3 signaling pathway.
Other Notes
Small volumes of STAT3 cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for STAT3 purchase
MBS669570 is a cell line targeting STAT3. Also known as STAT3 Leeporter Luciferase Reporter-NIH 3T3 Cell Line. Host: NIH 3T3 cells.
Additional Details
NCBI/Uniprot data below describe general gene information for STAT3. It may not necessarily be applicable to this product.
Protein Family
Signal transducer and activator of transcription
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.