TMB ELISA Substrate is a ready-to-use chromogenic substrate for detection of horseradish peroxidase (HRP) activity. HRP  can catalyze 3,3’,5,5’-tetramethylbenzidine (TMB) to yield a blue color, the maximal absorbance is at 370 nm or 620-652 nm;  however, upon addition of the stop solution, the solution turns to yellow and can be measured at 450 nm.

Storage

at 2~8°C in the dark for one year

Shipping

ice bag (4℃)