Size : 100mg
Synonyms: TMBZ-PS
| Description | TMB-PS (TMBZ-PS) is a chromogenic reagent for the detection of hydrogen peroxide e.g. in the enzymatic analysis of body fluids. |
| Synonyms | TMBZ-PS |
| Cell Research | Instructions I. Solution preparation 1. Stock solution: Dissolve TMB-PS in deionized water or an appropriate buffer, usually 1–10 mM stock solution. 2. Working solution: Dilute the stock solution to a working concentration, usually 0.1–1 mM, according to the experimental requirements. To enhance the reaction rate and sensitivity, it is recommended to use a solution containing hydrogen peroxide as the substrate solution. II. Operation steps 1. Enzyme-linked immunosorbent assay (ELISA): (1) Add specific antibodies or antigens to the wells of the ELISA plate and incubate. (2) Add an enzyme marker that binds to the target antibody or antigen. (3) Wash the plate with an appropriate buffer. (4) Add TMB-PS substrate solution (usually containing hydrogen peroxide) and incubate in the dark for a certain period of time (5–30 minutes) until a blue or purple reaction product is observed. (5) Stop reaction: Add an acidic solution (such as 2 M sulfuric acid or 1 M phosphoric acid) to terminate the reaction. The reactant changes from blue to yellow, which facilitates the reading of optical density. 2. Immunohistochemical staining: (1) Add TMB-PS substrate solution to tissue sections or cell cultures for staining. (2) Wash off excess dye with PBS and observe under a microscope. 3. Calibration and control: (1) Control group: Set up a group without substrate solution as a control to ensure the specificity of the substrate reaction. (2) Standard curve: A standard curve between optical density and concentration can be established using samples of known concentration for quantitative analysis. Notes: (1) Photosensitivity: TMB-PS is light-sensitive and should be avoided from prolonged exposure to strong light. (2) Storage conditions: TMB-PS should be stored in a dry, cool place, away from high temperatures and strong light, usually at -20°C. (3) pH range: TMB-PS performs best in an acidic environment of pH 3–5, but is also stable over a wider pH range. (4) Solubility: TMB-PS has good solubility in water, but it should be ensured that there are no undissolved solid particles. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 362.49 |
| Formula | C19H26N2O3S |
| Cas No. | 102062-36-2 |
| Smiles | Cc1cc(cc(C)c1N)-c1cc(C)c(NCCCS(O)(=O)=O)c(C)c1 |
| Relative Density. | 1.236 g/cm3 (Predicted) |
| Storage | Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 35 mg/mL (96.55 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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