VEGFR3/FLT4, BioAssay™ ELISA Kit (Rat) (Fms-related Tyrosine Kinase 4)

Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

Sample Type:
Cell culture supernates and serum.

Intended Use:
For quantitative detection of rat VEGFR3 in cell culture supernates and serum.

Sensitivity:
<10pg/ml

Range:
156-10,000pg/ml

Specificity:
Natural and recombinant rat VEGFR3

Test Principle:
This rat VEGFR3 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGFR3 has been precoated onto 96-well plates. Standards(NSO, Y25-D770) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat VEGFR3 amount of sample captured in plate.

Kit Components:
96-well plate precoated with rat VEGFR3 antibody, 1
Lyophilized recombinant rat VEGFR3 standard, 10ng/tube×2
Biotinylated rat VEGFR3 antibody, 130ul (dilution 1:100)
Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)
Sample diluent buffer, 30ml
Antibody diluent buffer, 12ml
ABC diluent buffer, 12ml
TMB color developing agent, 10ml
TMB stop solution, 10ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.