Size : 10g
Synonyms: VRBA, Violet Red Bile Agar
| Description | Violet Red Bile Agar (VRBA) is a selective and differential culture medium formulated for the enumeration of coliform bacteria in food, dairy, and water samples. The medium contains crystal violet and bile salts as selective inhibitors against Gram-positive organisms, while lactose fermentation by coliforms produces acid that reacts with the neutral red indicator, forming characteristic purple-red colonies. The rapid and reliable differentiation of lactose-fermenting enteric bacteria makes Violet Red Bile Agar a standard method in food safety testing and environmental microbiology. |
| In vitro | Violet Red Bile Agar is a selective medium for isolating and enumerating coliforms and Escherichia coli. In a study of fish pen slime, organisms mimicking E. coli on this agar were identified as Vibrio species based on metabolic and morphological characteristics (PMID: 4195607). |
| Synonyms | VRBA, Violet Red Bile Agar |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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