Vitamin B12 [68-19-9]
Katalog-Nummer HY-B0315-500mg
Size : 500mg
Marke : MedChemExpress
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Vitamin B12 is a vitamin. Vitamin B12 plays a key role in the normal functioning of the brain and nervous system, and for the formation of blood.
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Vitamin B12 Chemische Struktur
CAS. Nr. : 68-19-9
This product is a controlled substance and not for sale in your territory.
Based on 2 publication(s) in Google Scholar
Other Forms of Vitamin B12:
- Vitamin B12-13C7 In-stock
| Beschreibung |
Vitamin B12 is a vitamin. Vitamin B12 plays a key role in the normal functioning of the brain and nervous system, and for the formation of blood[1][2]. |
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| IC50 & Target |
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| In Vitro |
Vitamin B12 is one of the eight B vitamins. It is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid synthesis (especially odd chain fatty acids) and energy production. However, many (though not all) of the effects of functions of B12 can be replaced by sufficient quantities of folic acid (vitamin B9), since B12 is used to regenerate folate in the body. Most vitamin B12 deficiency symptoms are actually folate deficiency symptoms, since they include all the effects of pernicious anemia and megaloblastosis, which are due to poor synthesis of DNA when the body does not have a proper supply of folic acid for the production of thymine due to methyl trapping. When sufficient folic acid is available, all known B12 related deficiency syndromes normalize, save those narrowly connected with the vitamin B12-dependent enzymes Methylmalonyl Coenzyme A mutase, and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), also known as methionine synthase; and the buildup of their respective substrates (methylmalonic acid, MMA) and homocysteine.Coenzyme B12's reactive C-Co bond participates in three main types of enzyme-catalyzed reactions[1][2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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| Klinische Studie |
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| Molekulargewicht |
1355.37 |
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| Formel |
C63H88CoN14O14P |
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| CAS. Nr. |
68-19-9 |
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| Appearance |
Solid |
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| Color |
Brown to reddish brown |
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| SMILES |
N#[C-][Co+3]123445=C([C@H]([C@]6(C)CC(N)=O)CCC(N)=O)C(C)=C([C@](C)([C@@H]7CCC(N)=O)CC(N)=O)41=C7C=C(C(C)([C@@H]8CCC(N)=O)C)42=C8C(C)=C([C@@]([C@H]9CC(N)=O)(CCC(NC[C@H](OP([O-])(O[C@H]%10C([C@H](O[C@@H]%10CO)N%11C=44C%12=CC(C)=C(C)C=C%12%11)(03)O)=O)C)=O)C)[N-]3[C@@]9(03)[C@]56C |
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| Structure Classification |
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| Initial Source |
microorganisms |
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| Versand | Room temperature in continental US; may vary elsewhere. |
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| Speicherung |
4°C, protect from light *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light) |
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| Lösungsmittel & Löslichkeit |
In Vitro:
DMSO : 20.83 mg/mL (15.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 6.25 mg/mL (4.61 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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| Reinheit & Dokumentation |
Purity: 99.87% |
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| Verweise |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|
| H2O / DMSO | 1 mM | 0.7378 mL | 3.6890 mL | 7.3781 mL | 18.4451 mL |
| DMSO | 5 mM | 0.1476 mL | 0.7378 mL | 1.4756 mL | 3.6890 mL |
| 10 mM | 0.0738 mL | 0.3689 mL | 0.7378 mL | 1.8445 mL | |
| 15 mM | 0.0492 mL | 0.2459 mL | 0.4919 mL | 1.2297 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.