4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate is a phase transition temperature probe that may be used to study the effects of oxidative injury on enzyme activities. It has shown antibacterial efficacy against Gram-positive and Gram-negative bacteria, and anion radical scavenging abilities. 4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate also has been shown to have nuclear DNA binding properties. The analytical method for this compound is based on benzalkonium chloride (BAC). This compound has been studied as a model system for other pharmacological agents and its biological studies have shown that it may be effective in treating cancer tissues. 4-[3-(4-Iodophenyl)-2||Synonyms:|Water-soluble Tetrazolium 1; 4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene Disulfonate; 5-(2,4-Disulfophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium Inner Salt Sodium Salt (1:1)||CAS No:|150849-52-8||Molecular Formula:|C₁₉H₁₁IN₅NaO₈S₂||Molecular Weight:|651.35||Purity:|≥95% (HPLC)||Appearance:|White to off-white or yellow to brown powder||1H-NMR Spectrum:|Conforms to reference||Melting Point:|>280°C (dec.) (lit)||Storage and Stability:|Store at -20ºC. Stable for 6 months after receipt.