Orb1146916-24 | Zebrafish 5-HT(5-Hydroxytryptamine) ELISA Kit Clinisciences

Zebrafish 5-HT(5-Hydroxytryptamine) ELISA Kit

Catalog Number	orb1146916
Category	Assays and Kits
Description	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity	Zebrafish
Range	14.07-900 ng/mL
Sensitivity	3.97 ng/mL
Protein Length	2h
Alternative names	5HT; Serotonin; N-Methyl-Gamma
Note	For research use only
Sample Types	serum, plasma, tissue homogenates
Expiration Date	Please enquire.