AbGUARD PLATE STABILIZER
Katalog-Nummer MBS239094-1L
Size : 1L
Marke : MyBiosource
PLATE Accessory Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS239094MBS239094 is an accessory reagent targeting PLATE BLOCKING AND PROTECTION BUFFER. Also known as AbGUARD PLATE STABILIZER. More details
Product Overview
Product Name
PLATE BLOCKING AND PROTECTION BUFFER, Accessory Reagent
Full Product Name
AbGUARD PLATE STABILIZER
Form/Format
Ready To Use
Ready to use Plate Blocking and Protection Buffer - liquid
Ready to use Plate Blocking and Protection Buffer - liquid
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product is photosensitive and should be protected from light.
Shelf Life: 48 months from date of despatch.
Shelf Life: 48 months from date of despatch.
Intended Use
AbGuard Plate Stabilizer simultaneously stabilizes and preserves microwell plates coated with proteins or other biomolecules and blocks any free binding sites. It preserves the biological activity of bound molecules and prevents degradation, denaturation and leaching. It also blocks free binding sites without creating any interference.
This item is a weakly opaque solution containing proprietary stabilizing components. It does not contain azide, mercury or other toxic compounds. It is biologically inactive.
Instructions For Use||1. Adsorb the primary protein or other biomolecule to the surface of the microwell according to in-house procedures (typically wells are coated with 100ul of the biomolecule in a fresh carbonate buffer pH 9.6 overnight at 4 degree C or for 6 hours at room temperature).
2. Without emptying and washing of the plate, add into the coated wells 100-200ul of AbGuard Plate Stabilizer. Saturation is done at room temperature by incubation with AbGuard Plate Stabilizer for 10-15 minutes by gently stirring. Empty the plate by cautious inversion of the plate. DO NOT wash hereafter.
3. Dry the plate for long term storage. Recommended methods are:
Dry the plate in darkness at room temperature overnight. Do not cover the plate.
Dry the plate in darkness for 2 - 24 hours in a chamber with low humidity (less than 20%) until totally dry (electronic drying chamber or plastic box containing calcium sulphate or silica gel, etc.). Alternatively, dry for 3 hours at 37 degree C.
4. Immediately after drying, pack the stabilized microwell plate in an airtight, light-protecting and moisture-proof container or bag with a desiccant.
5. It is recommended to evaluate whether it is necessary to pre-wash stabilized plates prior to use.
Troubleshooting advice: it is recommended to introduce an extra wash step after coating, to remove unbound material, if unexpected results are obtained. Use standard washing methods of inverting the plate and tapping it on an absorbent paper, or use an automatic plate washer. It is essential that the coated area SHOULD NOT be allowed to dry before adding AbGuard Plate Stabilizer.
This item is a weakly opaque solution containing proprietary stabilizing components. It does not contain azide, mercury or other toxic compounds. It is biologically inactive.
Instructions For Use||1. Adsorb the primary protein or other biomolecule to the surface of the microwell according to in-house procedures (typically wells are coated with 100ul of the biomolecule in a fresh carbonate buffer pH 9.6 overnight at 4 degree C or for 6 hours at room temperature).
2. Without emptying and washing of the plate, add into the coated wells 100-200ul of AbGuard Plate Stabilizer. Saturation is done at room temperature by incubation with AbGuard Plate Stabilizer for 10-15 minutes by gently stirring. Empty the plate by cautious inversion of the plate. DO NOT wash hereafter.
3. Dry the plate for long term storage. Recommended methods are:
Dry the plate in darkness at room temperature overnight. Do not cover the plate.
Dry the plate in darkness for 2 - 24 hours in a chamber with low humidity (less than 20%) until totally dry (electronic drying chamber or plastic box containing calcium sulphate or silica gel, etc.). Alternatively, dry for 3 hours at 37 degree C.
4. Immediately after drying, pack the stabilized microwell plate in an airtight, light-protecting and moisture-proof container or bag with a desiccant.
5. It is recommended to evaluate whether it is necessary to pre-wash stabilized plates prior to use.
Troubleshooting advice: it is recommended to introduce an extra wash step after coating, to remove unbound material, if unexpected results are obtained. Use standard washing methods of inverting the plate and tapping it on an absorbent paper, or use an automatic plate washer. It is essential that the coated area SHOULD NOT be allowed to dry before adding AbGuard Plate Stabilizer.
Applications Tested/Suitable for PLATE BLOCKING AND PROTECTION BUFFER accessory reagent
ELISA (EIA)
Application Notes for PLATE BLOCKING AND PROTECTION BUFFER accessory reagent
ELISA: Maximum Dilution: Neat
Other Notes
Small volumes of PLATE BLOCKING AND PROTECTION BUFFER accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.