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Anti-TIE1 Polyclonal Antibody

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Katalog-Nummer NB-64-114410-100uL

Size : 100uL

Marke : Neo Biotech


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Anti-TIE1 Polyclonal Antibody

(Synonyms: tyrosine kinase with immunoglobulin-like and EGF-like domains 1, TIE, JTK14) Copy Product Info

Synonyms: tyrosine kinase with immunoglobulin-like and EGF-like domains 1, TIE, JTK14

Catalog No. TMAB-01838 Copy Product Info
Anti-TIE1 Polyclonal Antibody is a Rabbit antibody targeting TIE1. Anti-TIE1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-TIE1 Polyclonal Antibody is a Rabbit antibody targeting TIE1. Anti-TIE1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Synonymstyrosine kinase with immunoglobulin-like and EGF-like domains 1, TIE, JTK14
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. Sample:
A549 (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-TIE1 (TMAB-01838) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123 kDa
Observed band size: 123 kDa
2. Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TIE1 Polyclonal Antibody, Unconjugated (TMAB-01838) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Anti-TIE1 Polyclonal Antibody,Anti-TIE1 Polyclonal Antibody
Application
IF
IHC-Fr
IHC-P
WB
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
Tissue SpecificitySpecifically expressed in developing vascular endothelial cells.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundTIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Tie1
Antigen Species
Human
Gene Name
TIE1
Gene ID
7075
Protein Name
Tyrosine-protein kinase receptor Tie-1
Uniprot ID
P35590
Biology Area
Growth factor receptors,Endothelium,Mesoderm,Receptor Tyrosine Kinases,Endothelial Markers,Mesoderm
Function
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Chemical Properties
Molecular WeightTheoretical: 123 kDa. Actual: 123 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Keywords

JTK-14JTK 14
Related Tags: Anti-TIE1 Polyclonal Antibody molecular weight