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> CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

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Katalog-Nummer SM3010R

Size : 100Tests


CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

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SKU
SM3010R
CD15 mouse monoclonal antibody, clone MEM-158, PE
Size:
100 Tests
Conjugation:
PE
 
Specifications
Product Data
Clone Name MEM-158
Application FC
Recommended Dilution Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension.
The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity Human
Antibody Host Mouse
Isotype IgM
Clonality Monoclonal
Immunogen Human granulocytes.
Specificity The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
Negative Species: Porcine.
Buffer PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.
Label: PE
State: Liquid purified IgG fraction.
Label: Conjugated with R-Phycoerythrin under optimum conditions
Purification Size-exclusion chromatography.
Conjugation PE
Storage Store the antibody in the dark at 2-8°C.
Do Not Freeze!
Avoid prolonged exposure to light.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Synonyms Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1
Reference Data
Product Manuals
FAQs
  • Antibody and Western Standard
SDS

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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