Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> CD88 (CD88 Antigen, Anaphylatoxin Receptor, C5a Anaphylatoxin Chemotactic Receptor, C5aR, C5a-R, C5a Ligand, C5R1) (Biotin)

CD88 (CD88 Antigen, Anaphylatoxin Receptor, C5a Anaphylatoxin Chemotactic Receptor, C5aR, C5a-R, C5a Ligand, C5R1) (Biotin)

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Katalog-Nummer C2439-61E-Biotin-100ul

Size : 100ul

Marke : US Biological


C2439-61E-Biotin CD88 (CD88 Antigen, Anaphylatoxin Receptor, C5a Anaphylatoxin Chemotactic Receptor, C5aR, C5a-R, C5a Ligand, C5R1) (Biotin)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P21730
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin and frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 3H1740 (P12/1)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: C5aR-peptide: Met1-Asn31|Specificity: Recognizes human the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Does not inhibit the binding of C5a to its receptor.|Species Crossreactivity: Rhesus Monkey||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
C5aR-peptide: Met1-Asn31
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Does not inhibit the binding of C5a to its receptor.|Species Crossreactivity: Rhesus Monkey
References
1. Opperman, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain J. Immunol. 151: 3785 - 3794, 2. Opperman, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and anatagonize C5a biological activity. In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P: 955-956, 3. Werfel, T. et al. (1995) Binding of anti-C5a receptor (C5aR) antibodies to cells of clinically normal human skin. In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P.: 957-959, 4. Oppermann, M. (1995) Cluster report: (C5a receptor). In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P. 953-954, 5. Oppermann, M. and Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors Immunology. 82: 516-521, 6. Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin. J Immunol. 157: 1729-35.