CD9 Recombinant Monoclonal Mouse Antibody (2310.9) – Biotium Choice
Katalog-Nummer P015-1MG
Size : 200UL
Marke : Biotium
CD9 Recombinant Monoclonal Mouse Antibody (2310.9) - Biotium Choice
CD9 (clone 2310.9) is a validated recombinant mouse monoclonal antibody that recognizes the human tetraspanin protein CD9. The antibody belongs to the Biotium Choice list of select antibodies that have been developed and validated in-house for optimal performance.
Methanol fixed MCF7 cells stained with 5 ug/mL recombinant mouse anti-CD9 clone 2310.9 conjugated to CF®488A.
Western detection of human CD9 in MCF-7 cell and EV lysates using CD9 Recombinant Mouse Monoclonal Antibody (2310.9) followed by goat anti-mouse CF®680. 10 ug of MCF-7 cell lysate and 1 ug of MCF-7 EV lysate were loaded. The blot was imaged on a LI-COR Odyssey® infrared imaging system in the 700 channel.
Recombinant CD9(2310.9) antibody was conjugated to CF®488A and used to stain MCF-7 cell-derived EVs (left panel). EV staining was compared to the antibody alone in buffer (right panel). Staining was detected in the B525 (FITC) channel. 
Recombinant CD9(2310.9) antibody was conjugated to CF®405M and used to stain the surface of MCF-7 cells (blue). Unstained cells are shown in gray. Staining was detected in the V450 (Pacific Blue) channel. 
Recombinant CD9 (2310.9) antibody was conjugated to CF®488A and used to stain the surface of MCF-7 cells (green). Isotype control antibody conjugated to CF®488A is shown in gray. Staining was detected in the B525 (FITC) channel. 
Recombinant CD9(2310.9) antibody was conjugated to CF®568 and used to stain the surface of MCF-7 cells (orange). Isotype control antibody conjugated to CF®568 is shown in gray. Staining was detected in the Y585 (PE) channel. 
Recombinant CD9 (2310.9) antibody was conjugated to CF®583R and used to stain the surface of MCF-7 cells (red). Unstained cells are shown in gray. Staining was detected in the Y610 (mCherry) channel. 
Recombinant CD9(2310.9) antibody was conjugated to CF®647Plus and used to stain the surface of MCF-7 cells (pink). Unstained cells are shown in gray. Staining was detected in the R660 (APC) channel. 
Recombinant CD9(2310.9) antibody was conjugated to APC and used to stain the surface of MCF-7 cells (pink). Isotype control antibody conjugated to APC is shown in gray. Staining was detected in the R660 (APC) channel. 
Recombinant CD9(2310.9) antibody was conjugated to CF®700 and used to stain the surface of MCF-7 cells (teal). Isotype control antibody conjugated to CF®700 is shown in gray. Staining was detected in the R712 (APC-700) channel. 
Recombinant CD9(2310.9) antibody was conjugated to RPE-Astral™775 and used to stain the surface of MCF-7 cells (teal). Isotype control antibody conjugated to RPE-Astral™775 is shown in gray. Staining was detected in the Y763 channel.Product Description
CD9 (clone 2310.9) is a recombinant mouse monoclonal antibody that recognizes the human tetraspanin protein CD9. This antibody belongs to the Biotium Choice list of select antibodies that have been developed and validated in-house for optimal performance. This antibody has been validated in flow cytometry, immunofluorescence microscopy, and in western blot. The antibody is available unlabeled or conjugated to CF® Dyes and Astral™ tandem dyes.
CD9 is a type IV transmembrane glycoprotein with four transmembrane domains. CD9 on pre-B cells may play a role in cell-cell adhesion. In addition, CD9 may play a role in signal transduction mediated by interaction with low molecular weight GTP binding proteins. CD9 is expressed on early B cells, eosinophils, basophils and activated T cells and is a major component of the platelet cell surface. It is also expressed on most non-T acute lymphoblastic leukemia cells and on some acute myeloid and chronic lymphoid leukemia.
Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.
As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody.
Exosome Stains and Antibodies
Biotium also offers ExoBrite™ CD9 Flow Antibody and ExoBrite™ CD9 Western Antibody that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting. Learn more about our products for exosome research.
Discover Sharper Signals and Unmatched Panel Flexibility with Biotium Choice Antibodies
Powered by CF® Dyes and Astral Leap™ Tandem Dyes
Biotium Choice antibodies are carefully curated and validated in-house to offer exceptional signal-to-noise. Labeled with our advanced CF® Dyes and Astral Leap™ tandem dyes, they are our top-recommended antibodies for flow cytometry and other applications.
Biotium Choice Antibody Features
- Robust and validated clones against common targets
- Developed and optimized for flow cytometry and other applications
- Conjugated to bright, photostable CF® Dyes for superior signal and clarity
- Also available with Astral Leap™ tandem dyes for expanding multiplexing
- New antibody clones and dye conjugates continuously in development
View our full catalog of Biotium Choice antibodies
Identifying Biotium validated antibodies
Biotium scientists validate and optimize select antibodies in our catalog, marked with a check mark next to the product name. This symbol indicates the antibody is highly recommended for the specified application(s).
Look for this symbol next to the product name on product pages and search results
| Conjugation | Ex/Em | Conc. | Size | Catalog No. | Dye Features |
|---|---|---|---|---|---|
| CF®405M | 416/452 nm | 100 ug/mL | 25 tests (125 uL) | P015-405M-125 | CF®405M Features |
| 100 tests (500 uL) | P015-405M-500 | ||||
| CF®488A | 490/516 nm | 100 ug/mL | 25 tests (125 uL) | P015-488A-125 | CF®488A Features |
| 100 tests (500 uL) | P015-488A-500 | ||||
| CF® 568 | 562/584 nm | 100 ug/mL | 25 tests (125 uL) | P015-568-125 | CF®568 Features |
| 100 tests (500 uL) | P015-568-500 | ||||
| CF® 583R | 585/609 nm | 100 ug/mL | 25 tests (125 uL) | P015-583R-125 | CF®583R Features |
| 100 tests (500 uL) | P015-583R-500 | ||||
| RPE-Astral™616 | 496, 546, 566/617 nm | 100 ug/mL | 25 tests (125 uL) | P015-R616-125 | RPE-Astral™616 Features |
| 100 tests (500 uL) | P015-R616-500 | ||||
| CF®647Plus | 652/668 nm | 100 ug/mL | 25 tests (125 uL) | P015-647P-125 | |
| 100 tests (500 uL) | P015-647P-500 | ||||
| CF®700 | 696/721 nm | 100 ug/mL | 25 tests (125 uL) | P015-700-125 | CF®700 Features |
| 100 tests (500 uL) | P015-700-500 | ||||
| RPE-Astral™775 | 496, 546, 565/774 nm | 100 ug/mL | 25 tests (125 uL) | P015-R775-125 | RPE-Astral™775 Features |
| 100 tests (500 uL) | P015-R775-500 | ||||
| APC-Astral™813 | 633, 638/813 nm | 100 ug/mL | 25 tests (125 uL) | P015-A813-125 | APC-Astral™813 Features |
| 100 tests (500 uL) | P015-A813-500 | ||||
| R-PE | 496, 546, 565/576 nm | 50 ug/mL | 25 tests (125 uL) | P015-RPE-125 | |
| 100 tests (500 uL) | P015-RPE-500 | ||||
| APC | 651/660 nm | 20 ug/mL | 25 tests (125 uL) | P015-APC-125 | |
| 100 tests (500 uL) | P015-APC-500 | ||||
| Purified (BSA-free) | N/A | 2 mg/mL | 1 mg (500 uL) | P015-1MG | |
| 5 mg (2.5 mL) | P015-5MG |
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