FastPfu Fly DNA Polymerase Enzyme

FastPfu Fly DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase with an extension rate of up to 6 kb/min. Amplification of genomic DNA fragment up to 15 kb. Amplification of plasmid DNA fragment up to 20 kb.

Contents:

Component	250 U	500 U	3 kU
FastPfu Fly DNA Polymerase	1 × 250 U	1 × 500 U	6 × 500 U
2X FastPfu Fly Reaction Mix	3 × 1 ml	5 × 1.2 ml	30 × 1.2 ml
50 mM MgSO4	200 µl	400 µl	1 ml
PCR Stimulant	200 µl	400 µl	1 ml
6X DNA Loading Buffer	500 µl	1 ml	2 × 1 ml

Target	FastPfu Fly DNA Polymerase
Tested Applications	PCR
Conjugation	Unconjugated
Storage	Store at -20°C for up to 2 years. Avoid repeated freeze/thaw cycles.
Buffer	Contains glycerol. The exact formulation is proprietary.
Concentration	2.5 U/µl
Availability	Shipped within 10-20 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
Directions for use	Reaction Components: Component Volume Final Concentration Template Variable as required Forward Primer (10 µM) 1 µl 0.2 µM Reverse Primer (10 µM) 1 µl 0.2 µM 2X FastPfu Fly Reaction Mix 25 µl 1X FastPfu Fly DNA Polymerase 1 µl 2.5 U Nuclease-Free ddH2O Variable N/A Total Volume 50 µl N/A Suggested Reaction Conditions: Template Quantity Genomic DNA 10-100 ng Genomic DNA Plasmid DNA 1-30 ng Plasmid DNA cDNA 1-2 µl cDNA from RT reaction (50-500 ng RNA for RT reaction) Thermal Cycling Conditions: Number of Cycles Temperature Time 1 cycle 95 °C 2 min 30-35 cycles 95 °C 20 seconds TM - 5 °C 20 seconds 72 °C 6 kb/min 1 cycle 72 °C 5 min Notes: The PCR Stimulant can be used to optimize the amplification of complex templates or high GC/AT templates. The recommended working concentration is 0.5X - 2X (concentration of the provided stock solution is 5X). It is recommended to add the FastPfu Fly DNA Polymerase last in the reaction system. For GC-rich templates, the recommended denaturation temperature is 98 °C. If precipitates in the FastPfu Fly Reaction Mix are observed, warm to 37 °C in a water bath and mix before use.

Research Articles on FastPfu Fly DNA Polymerase