Herpes Simplex Virus II, IgM (HSV II), ELISA Kit, BioAssay™

ELISA Kit for Antibody IgM to Herpes Simplex Virus (HSV) Type II is an in vitro enzyme immunoassay for the detection of Anti-HSV antibodies IgM in human serum or plasma. It is for diagnosis of early infection and epidemic survey.

Principle:
This kit uses capture ELISA method to detect anti- HSV antibodies IgM in serum or plasma. The mouse anti-human IgM (mu chain) antibody is coated on the multi-wells plate. The HRP conjugated recombinant HSV antigen serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-HSV antibodies IgM in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of HSV infection.

Kit Components:
1. Antibody Coated Microwell Plate, 1x96 wells
2. Enzyme Conjugant
3. Negative Control Serum
4. Positive Control Serum
5. Concentrated Wash Buffer (20X)
6. Substrate A
7. Substrate B
8. Stop Solution

Storage and Stability:
Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.