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> HLAF (HLA-F) (NM_001098479) Human Over-expression Lysate

HLAF (HLA-F) (NM_001098479) Human Over-expression Lysate

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Katalog-Nummer LY420587

Size : 100ug


HLAF (HLA-F) (NM_001098479) Human Over-expression Lysate

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SKU
LY420587
Transient overexpression lysate of major histocompatibility complex, class I, F (HLA-F), transcript variant 1 (as WB positive control)
 

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Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212120
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001098479, NP_001091949
Synonyms CDA12; HLA-5.4; HLA-CDA12; HLAF
Predicted MW 50.44 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001091949
Locus ID 3134
Cytogenetics 6p22.1
Protein Families WB
Protein Pathways Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Type I diabetes mellitus, Viral myocarditis
Product Manuals
  • Western Blot Protocols
FAQs
  • Over-expression Cell Lysate
SDS
SKU Description Size
LC420587 Lyophilized HLA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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