5'C↓TNAG3'
3'GANT↑C5'

HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer. Includes universal Tango buffer for double-digestions

Concentration:
~10U/ul

Isoschizomers:
BstDEI, DdeI

Specifications:
Methylation Sensitivity: Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive
Enzyme: HpyF3I (DdeI)
Compatible Buffer: 10x Buffer Tango
Sensitive to Heat Inactivation: Yes
Optimal Reaction Temperature: 37°C
Type IIS RE: No

Unit Definition:
One unit is defined as the amount of HpyF3I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer.

Storage Buffer:
HpyF3I is supplied in: 10mM Tris-HCl, (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.

Thermal Inactivation:
HpyF3I is inactivated by incubation at 65°C for 20 min.

Applications:
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.