Quantitative, sandwich-based, glass slide antibody array to detect 30 human angiogenesis protein biomarkers.
Human Angiogenesis Array Q2
Human Angiogenesis Array Q2
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 30 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Activin A
| AgRP
| Angiogenin
| Angiopoietin-2
| ANGPTL4
|
bFGF
| ENA-78 (CXCL5)
| GRO alpha/beta/gamma
| HB-EGF
| HGF
|
IFN-gamma
| IGF-1
| IL-1 alpha (IL-1 F1)
| IL-2
| IL-6
|
IL-8 (CXCL8)
| IL-17A
| IP-10 (CXCL10)
| Leptin
| LIF
|
MCP-1 (CCL2)
| PDGF-BB
| PLGF
| RANTES (CCL5)
| TGF beta 1
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| Thrombopoietin (TPO)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Angiogenesis Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-ANG-2.gal)
Excel (QAH-ANG-2_Analysis_Tool.xls)
citations
CoQ10 reduces glioblastoma growth and infiltration through proteome remodeling and inhibition of angiogenesis and inflammation
- Species:
- Human
- Sample Type:
- U251 cell
- Year:
- 2022
- Journal Name:
- Cellular Oncology
Predictive value of angiogenic proteins in patients with metastatic melanoma treated with bevacizumab monotherapy
- Species:
- Human
- Sample Type:
- Serum (Biomarker of angiogenic proteins with metastatic melanoma and bevacizumab)
- Year:
- 2019
- PubMed ID:
- 30225999
The role of HMGB1 in BMSC transplantation for treating MODS in rats
- Species:
- Human
- Sample Type:
- Conditioned Media (cu;ltured BMSC cells)
- Year:
- 2018
- Journal Name:
- Cell and Tissue Research
(2014) Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells
- Species:
- Human
- Sample Type:
- Cell Lysate (Microvesicles and MSC parents)
- Year:
- 2015
- Journal Name:
- Plos One
- PubMed ID:
- 25514634
Differential expression of steroid 5-reductase isozymes and association with disease severity and angiogenic genes predict their biological role in prostate cancer
- Species:
- Human
- Sample Type:
- Cell Lysate; Serum
- Year:
- 2010