Human CD33 Antibody : LE/AF
Katalog-Nummer OASB01082
Size : 0.5mg
Marke : Aviva Systems Biology
Human CD33 Antibody : LE/AF (OASB01082)
| Datasheets/Manuals | Printable datasheet for Human CD33 Antibody : LE/AF (OASB01082) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | WM53 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, ICC, IP, WB, BL |
| Additional Information | Description: CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. |
| Reconstitution and Storage | Store at -20C |
| Concentration | 0.5 mg/mL |
| Specificity | CD33 |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody Fluorescein conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate R-phycoerythrin-Cyanine 5.5 conjugate <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunoprecipitation, Western blotting, Immunohistochemistry (frozen sections) |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5) conjugates aresupplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C Conjugated forms should not be frozen and should be protected from prolonged exposure to light Aliquot and freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Eachreagent is stable for the period shown on the bottle label if stored as directed. |
| Reference | 1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford 2. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd,eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford 3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD33 Section, Academic Press, New York, p. 213 4. Freeman, S.D., S. Kelm, E.K. Barber, and P.R. Crocker. 1995. Blood 85:2005. |
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| Gene Symbol | CD33 |
| Gene Full Name | Myeloid cell surface antigen CD33 |
| Alias Symbols | p67, SIGLEC3, SIGLEC-3 |
| NCBI Gene Id | 945 |
| Protein Name | myeloid cell surface antigen CD33 |
| Uniprot ID | P20138 |
| Protein Accession # | NP_001076087.1 |
| Nucleotide Accession # | NM_001082618.1 |
