Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> IgD, Heavy Chain

IgD, Heavy Chain

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Katalog-Nummer I1895-03J-500ug

Size : 500ug

Marke : US Biological


I1895-03J IgD, Heavy Chain

Clone Type
Polyclonal
Host
goat
Source
human
Swiss Prot
P01880
Isotype
IgG
Grade
Affinity Purified
Applications
E FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA and Flow Cytometry. Other application, though not tested is Immunohistochemistry (ref 1-4). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in borate buffered saline, pH 8.2.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Human IgD|Specificity: Recognizes heavy chain of human IgD. Has been cross-absorbed against human IgM, IgA and IgG. May react with IgD from other species.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human IgD
Form
Supplied as a liquid in borate buffered saline, pH 8.2.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes heavy chain of human IgD. Has been cross-absorbed against human IgM, IgA and IgG. May react with IgD from other species.
References
1. Pospisil R, Silverman GJ, Marti GE, Aruffo A, Bowen MA, Mage RG. CD5 is a potential selecting ligand for B-cell surface immunoglobulin: a possible role in maintenance and selective expansion of normal and malignant B cells. Leuk Lymphoma. 2000;36:353-65. (IHC-FS)|2. Hirbod T, Kaldensjö T, Broliden K. In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. PLoS One. 2011;6(9):e25551. (IHC-FS)|3. Rakhmanov M, Sic H, Kienzler A, Fischer B, Rizzi M, Seidl M, et al. High levels of SOX5 decrease proliferative capacity of human B cells, but permit plasmablast differentiation. PLoS One. 2014;9(6):e100328. (IHC-FS)|4. Cols M, Barra CM, He B, Puga I, Xu W, Chiu A, et al. Stromal endothelial cells establish a bidirectional crosstalk with chronic lymphocytic leukemia cells through the TNF-related factors BAFF, APRIL, and CD40L. J Immunol. 2012;188:6071-83. (IHC-FS)|5. Mongini PK, Blessinger C, Posnett DN, Rudich SM. Membrane IgD and membrane IgM differ in capacity to transduce inhibitory signals within the same human B cell clonal populations. J Immunol. 1989;143:1565-74. (FC)|6. Kumagai M, Coustan-Smith E, Murray DJ, Silvennoinen O, Murti KG, Evans WE, et al. Ligation of CD38 suppresses human B lymphopoiesis. J Exp Med. 1995;181:1101-10. (FC)|7. Klein U, Rajewsky K, Küppers R. Human immunoglobulin (Ig)M+IgD+ peripheral blood B cells expressing the CD27 cell surface antigen carry somatically mutated variable region genes: CD27 as a general marker for somatically mutated (memory) B cells. J Exp Med. 1998;188:1679-89. (FC)|8. van der Loo JC, Hanenberg H, Cooper RJ, Luo F, Lazaridis EN, Williams DA. Nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mouse as a model system to study the engraftment and mobilization of human peripheral blood stem cells. Blood. 1998;92:2556-70. (FC)|9. Bräuninger A, Goossens T, Rajewsky K, Küppers R. Regulation of immunoglobulin light chain gene rearrangements during early B cell development in the human. Eur J Immunol. 2001;31:3631-7. (FC)|10. Fournillier A, Freida D, Defrance T, Merle P, Trépo C, Inchauspé G. Analysis of B-lymphocyte differentiation in patients infected with hepatitis C virus. J Med Virol. 2004;72:566-74. (FC)|11. Johansen F, Baekkevold ES, Carlsen HS, Farstad IN, Soler D, Brandtzaeg P. Regional induction of adhesion molecules and chemokine receptors explains disparate homing of human B cells to systemic and mucosal effector sites: dispersion from tonsils. Blood. 2005;106:593-600. (FC)|12. Dryer RL, Covey LR. Use of chromatin immunoprecipitation (ChIP) to detect transcription factor binding to highly homologous promoters in chromatin isolated from unstimulated and activated primary human B cells. Biol Proced Online. 2006;8:44-54. (FC)|13. Sira MM, Yoshida T, Takeuchi M, Kashiwayama Y, Futatani T, Kanegane H, et al. A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells. Int Immunol. 2009;21:1013-23. (FC)|14. Petrovas C, Yamamoto T, Gerner MY, Boswell KL, Wloka K, Smith EC, et al. CD4 T follicular helper cell dynamics during SIV infection. J Clin Invest. 2012;122:3281-94. (FC)|15. Pallikkuth S, Micci L, Ende ZS, Iriele RI, Cervasi B, Lawson B, et al. Maintenance of intestinal Th17 cells and reduced microbial translocation in SIV-infected rhesus macaques treated with interleukin (IL)-21. PLoS One. 2013;9(7):e1003471. (FC)|16. Yammani RD, Haas KM. Primate B-1 cells generate antigen-specific B cell responses to T cell-independent type 2 antigens. J Immunol. 2013;190:3100-8. (FC)|17. Neumann B, Klippert A, Raue K, Sopper S, Stahl-Hennig C. Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry. J Leukoc Biol. 2015;97:19-30. (FC)|18. Joseph AM, Babcock GJ, Thorley-Lawson DA. Cells expressing the Epstein-Barr virus growth program are present in and restricted to the naive B-cell subset of healthy tonsils. J Virol. 2000;74:9964-71. (Sep)|