LC3B antibody [GT3612]
![Anti-LC3B antibody [GT3612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632501](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFg2MzI1MDEvR1RYNjMyNTAxXzQyMjYxXzIwMTUxMjMwX0lGQV93XzIzMDYxMjAyXzcxMi53ZWJw.webp)
LC3B antibody [GT3612] detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LC3B protein stained by LC3B antibody [GT3612] (GTX632501) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
![Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFg2MzI1MDEvR1RYNjMyNTAxXzQyMTU2XzIwMTUxMTA1X1dCX0Jfd18yMzA2MTIwMl8xNDAud2VicA==.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.
![Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFg2MzI1MDEvR1RYNjMyNTAxXzQyMjYxXzIwMTUxMTA1X1dCX1RoYXBzaWdhcmdpbl93XzIzMDYxMjAyXzkxNi53ZWJw.webp)
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.
![Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFg2MzI1MDEvR1RYNjMyNTAxXzQyMjYxXzIwMTUxMjMxX1dCX2NobG9yb3F1aW5lX3dfMjMwNjEyMDJfMTQ2LndlYnA=.webp)
Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.