Maltose phosphorylase antibody (Peroxidase)
Katalog-Nummer orb344261-100ug
Size : 100ug
Marke : Biorbyt
Contact local distributor :
Telefonnummer : +1 850 650 7790
Maltose phosphorylase antibody (Peroxidase)
Catalog Number: orb344261
| Catalog Number | orb344261 |
|---|---|
| Category | Antibodies |
| Description | Maltose phosphorylase antibody (Peroxidase) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IP, WB |
| Reactivity | Bacteria |
| Isotype | IgG |
| Immunogen | Maltose Phosphorylase [E.coli] |
| Concentration | 1 mg/mL |
| Dilution range | ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | Maltose Phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase [E.coli]. Cross reactivity against Maltose Phosphorylase from other sources is unknown. |
| Conjugation | HRP |
| UniProt ID | B2GEX2 |
| NCBI | YP_001842841.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | goat anti-Maltose Phosphorylase Antibody HRP Conju |
| Note | For research use only |
| Application notes | Anti-Maltose Phosphorylase has a working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Biorbyt Goat anti Maltose Phosphorylase antibody was used to detect Maltose Phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).