C-Series Mouse Apoptosis Antibody Array 1 Kit. Detects 17 phosphorylated or cleaved mouse Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Mouse Apoptosis Signaling Pathway Array C1
Mouse Apoptosis Signaling Pathway Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 17 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Apoptosis, HER/ErbB Signaling, HIF-1 alpha Signaling, IGF Signaling, JAK/STAT Signaling, MAPK Signaling, mTOR Signaling, PI3K-AKT Signaling, TGF-beta Signaling, p53 Signaling, NFkB Signaling, PKC Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| AKT (P-Ser473)
| ATM (P-Ser1981)
| BAD (P-Ser112)
| Caspase-3 (P-Asp175)
| Caspase-7 (P-Asp198)
|
| CHK1 (P-Ser296)
| eIF-2a (P-Ser52)
| ERK1/2 (P-Thr202)
| HSP27 (P-Ser82)
| IKBa (P-Ser32)
|
| JNK (P-Thr183)
| NF-kB P65 (P-Ser536)
| p27 (P-Thr198)
| p38 (P-Thr180/Tyr182)
| P53 (P-Ser15)
|
| SMAD2 (P-Ser245)
| TAK1 (P-Ser412)
| |||
Typical Results
Kit Components
- Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Stigmasterol isolated from Azadirachta indica flowers attenuated glutamate-induced neurotoxicity via downregulation of the Cdk5/p35/p25 signaling pathway in the HT-22 cells
- Species:
- Mouse
- Sample Type:
- HT-22 cell
- Year:
- 2023
- Journal Name:
- Phytomedicine
Hepatocyte survival and proliferation by fibroblast growth factor 7 attenuates liver inflammation, and fibrogenesis during acute liver injury via paracrine mechanisms
- Species:
- Mouse
- Sample Type:
- liver
- Year:
- 2023
- Journal Name:
- Biomedicine & Pharmacotherapy
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- Year:
- 2022
- Journal Name:
- Communications biology
- PubMed ID:
- 35058552
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- Year:
- 2022
- Journal Name:
- Neuroscience Letters
- PubMed ID:
- 35490904
Gold nanoparticles loaded TNF-α and CALNN peptide as a drug delivery system and promising therapeutic agent for breast cancer cells
- Species:
- Mouse
- Sample Type:
- AN3 cell-Xenograft
- Year:
- 2022
- Journal Name:
- Materials Technology
Neuroprotective effects of ergosterol against TNF-α-induced HT-22 hippocampal cell injury
- Species:
- Mouse
- Sample Type:
- HT-22 cell
- Year:
- 2022
- Journal Name:
- Biomedicine & Pharmacotherapy
Metformin Inducing the Change of Functional and Exhausted Phenotypic Tumor-Infiltrated Lymphocytes and the Correlation with JNK Signal Pathway in Triple-Negative Breast Cancer
- Species:
- Mouse
- Sample Type:
- 4T1 cell
- Year:
- 2022
- Journal Name:
- Breast Cancer-Targets and Therapy
- PubMed ID:
- 36482884
The insect molting hormone 20-hydroxyecdysone protects dopaminergic neurons against MPTP-induced neurotoxicity in a mouse model of Parkinson's disease
- Year:
- 2020
- Journal Name:
- Free Radic Biol Med
- PubMed ID:
- 32745769
Needle-Free Injection of Exosomes Derived from Human Dermal Fibroblast Spheroids Ameliorates Skin Photoaging
- Year:
- 2019
- Journal Name:
- Acs Nano
- PubMed ID:
- 31449388
NLRP6 Deficiency in CD4 T Cells Decreases T Cell Survival Associated with Increased Cell Death
- Year:
- 2019
- Journal Name:
- Journal of Immunology
- PubMed ID:
- 31152078