RayBio® C-Series Mouse Cytokine Antibody Array 2 Kit. Detects 32 Mouse Cytokines. Suitable for all liquid sample types.
Mouse Cytokine Array C2
Mouse Cytokine Array C2
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 32 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
6Ckine (CCL21)
| CTACK (CCL27)
| Eotaxin-1 (CCL11)
| GCSF
| GM-CSF
|
IL-2
| IL-3
| IL-4
| IL-5
| IL-6
|
IL-9
| IL-10
| IL-12 p40/p70
| IL-12 p70
| IL-13
|
IL-17A
| IFN-gamma
| KC (CXCL1)
| Leptin
| MCP-1 (CCL2)
|
MCP-5
| MIP-1 alpha (CCL3)
| MIP-2
| MIP-3 beta (CCL19)
| RANTES (CCL5)
|
SCF
| TNF RI (TNFRSF1A)
| TARC (CCL17)
| TIMP-1
| TNF alpha
|
Thrombopoietin (TPO)
| VEGF-A
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
CTLA4 aptamer delivers STAT3 siRNA to tumor-associated and malignant T cells
- Species:
- Mouse
- Sample Type:
- Conditioned Media (cell supernatants)
- Year:
- 2014
- Journal Name:
- Journal of Clinical Investigation
The role of aquaporin 4 in apoptosis after intracerebral hemorrhage
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Brain Lysates)
- Year:
- 2014
- Journal Name:
- Journal of Neuroinflammation
Cyclin D1 activity regulates autophagy and senescence in the mammary epithelium
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Mammary gland built organoid)
- Year:
- 2012
- Journal Name:
- Cancer Research
Transcription repressor Bach2 is required for pulmonary surfactant homeostasis and alveolar macrophage function.
- Species:
- Mouse
- Sample Type:
- BAL fluid
- Year:
- 2013
- Journal Name:
- Journal of Experimental Medicine
- PubMed ID:
- 24127487
Immunomodulatory activities of mushroom sclerotial polysaccharides.
- Species:
- Mouse
- Sample Type:
- Serum (activation of innate immune cells and T-helper cells in normal and athymic BALB/c mice by three mushroom sclerotial polysaccharide)
- Year:
- 2011
- Journal Name:
- Food Hydrocolloids
1,2,3,4,6-penta-O-galloyl-?-D-glucopyranose increases a population of T regulatory cells and inhibits IgE production in ovalbumin-sensitizedmice
- Species:
- Mouse
- Sample Type:
- Serum (drug treated mice and Treg cells)
- Year:
- 2015
- Journal Name:
- International Immunopharmacology
- PubMed ID:
- 25737197
Placenta Suppresses Experimental Autoimmune Hypophysitis through Soluble TNF Receptor 1
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Placental and Muscle protein extracts)
- Year:
- 2012
- Journal Name:
- Journal of Autoimmunity
Identification of an IL-17-producing NK1.1neg iNKT cell population involved in airway neutrophilia
- Species:
- Mouse
- Sample Type:
- Conditioned Media (NK1.1+ sorted cells treated with alphaGalser)
- Year:
- 2007
- Journal Name:
- Journal of Experimental Medicine
Suppression of mucin 2 promotes interleukin-6 secretion and tumor growth in an orthotopic immune-competent colon cancer animal model
- Species:
- Mouse
- Sample Type:
- Conditioned Media (CT26 colon carcinoma cells)
- Year:
- 2014
- Journal Name:
- Oncology Reports
Induced Pluripotent Stem Cell-derived Neural Stem Cells Transduced with Baculovirus Encoding CD40 Ligand for Immunogene Therapy in Mouse Models of Breast Cancer
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Breast Cancer (4TI) cell line incubated with neural stem cells)
- Year:
- 2014
- Journal Name:
- Human Gene Therapy