Quantibody® Mouse Cytokine Array 1 Kit. Detects 20 Mouse Cytokines. Suitable for all liquid sample types.
Mouse Cytokine Array Q1
Mouse Cytokine Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
|
IL-12 p70
| IL-13
| IL-17A
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-9
| KC (CXCL1)
|
MCP-1 (CCL2)
| M-CSF
| RANTES (CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-CYT-1.gal)
Excel (QAM-CYT-1_Analysis_Tool.xls)
citations
Pediococcus acidilactici Y01 reduces HFD-induced obesity via altering gut microbiota and metabolomic profiles and modulating adipose tissue macrophage M1/M2 polarization
- Year:
- 2025
- Journal Name:
- Food & Function
- PubMed ID:
- 39699275
Tumor necrosis factor from leukemic environment stimulates hematopoietic stem/progenitor cells toward megakaryocyte/myeloid lineage bias
- Species:
- Mouse
- Sample Type:
- Serum (MA9 leukemic mice)
- Year:
- 2026
- Journal Name:
- Institutional Repositories DataBase (IRDB)
Neutrophil peptide 1 accelerates the clearance of degenerative axons during Wallerian degeneration by activating macrophages after peripheral nerve crush injury
- Year:
- 2024
- Journal Name:
- Neural Regeneration Research
- PubMed ID:
- 38103249
TREM2hi resident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis
- Species:
- Mouse
- Sample Type:
- heart tissue
- Year:
- 2023
- Journal Name:
- Nature Metabolism
Nogo-B mediates endothelial oxidative stress and inflammation to promote coronary atherosclerosis in pressure-overloaded mouse hearts
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Redox biology
Intraperitoneal infection of A/J and CD1 mice with coxsackievirus B2 and its mutants
- Species:
- Mouse
- Sample Type:
- Serum (pooled sera)
- Year:
- 2025
- Journal Name:
- Acta Virologica
Monophosphoryl Lipid A-2,4-Dinitrophenylamine Conjugates Are Potent Adjuvants for Carbohydrate and Protein Vaccines
- Species:
- Mouse
- Sample Type:
- Serum (pooled serum)
- Year:
- 2025
- Journal Name:
- JACS Au
- PubMed ID:
- 40443885
Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis
- Species:
- Mouse
- Sample Type:
- Serum (control or OMV-vaccinated BALB/c mice)
- Year:
- 2024
- Journal Name:
- Frontiers in Microbiology
- PubMed ID:
- 38533334
Sleep deprivation-induced sympathetic activation promotes pro-tumoral macrophage phenotype via the ADRB2/KLF4 pathway to facilitate NSCLC metastasis
- Species:
- Mouse
- Sample Type:
- Serum (sleep-deprived tumor-bearing mice)
- Year:
- 2025
- Journal Name:
- iScience
- PubMed ID:
- 40276761
Investigation of the Impact of Tryptophan-Metabolizing Enzymes and Kynurenic Acid on Antibody-Mediated Glomerulonephritis
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (culture supernatants from neutrophils on IC-coated plates)
- Year:
- 2025
- Journal Name:
- FASEB Journal
- PubMed ID:
- 40985497
